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Saccharopolyspora spinosa strain, application of saccharopolyspora spinosa strain and method for preparing spinosad

A technology of Saccharopolyspora spinosa and spinosad, applied in the field of preparation of spinosad, can solve the problems of no industrial application value, poor bacterial strain performance, etc., and achieve good industrial application value and spinosad capability strong effect

Active Publication Date: 2013-01-23
牡丹江佰佳信生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the spinosad fermentation technology in my country is still immature. The root cause is that the performance of the strain is poor. The concentration of spinosad in the fermentation broth can only reach 2g / L, which does not have industrial application value.

Method used

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  • Saccharopolyspora spinosa strain, application of saccharopolyspora spinosa strain and method for preparing spinosad
  • Saccharopolyspora spinosa strain, application of saccharopolyspora spinosa strain and method for preparing spinosad
  • Saccharopolyspora spinosa strain, application of saccharopolyspora spinosa strain and method for preparing spinosad

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preparation example Construction

[0027] The method for preparing spinosad provided by the present invention comprises the following steps: fermenting Saccharopolyspora spinosa 3460 to obtain a fermentation liquid containing spinosad.

[0028] In a preferred embodiment, the method for preparing spinosad of the present invention comprises the following steps:

[0029] (a) fermenting Saccharopolyspora spinosa 3460 of the present invention, or its mutant strain, to obtain a fermentation broth containing spinosad;

[0030] (b) Isolating spinosad from the fermentation broth.

[0031] Preferably, the method of the present invention includes the following steps: before fermentation, seed culture is carried out to obtain seed culture liquid.

[0032] In a preferred embodiment, the isolation of spinosad is carried out by:

[0033] (a) the fermentation broth containing spinosad is mixed with an organic solvent;

[0034] (b) solid-liquid separation to obtain spinosad extract.

[0035] This method of directly mixing t...

Embodiment 1

[0112] (1) Shake flask fermentation

[0113] Put 40mL of fermentation medium into a 250mL Erlenmeyer flask, and sterilize it for later use. Insert 4 milliliters of seed culture liquid into the bottle, carry out shaker fermentation culture, temperature is 28 ℃, shaker speed is 220rpm, cultivate 8 days, the relative humidity of fermentation environment is 56%, obtain about 35mL fermentation liquid.

[0114] Composition of the fermentation medium:

[0115] The final concentration of soluble starch is 60g / L,

[0116] The final concentration of glucose is 10g / L,

[0117] The final concentration of soybean meal powder is 30g / L,

[0118] The final concentration of corn steep liquor is 10g / L,

[0119] The final concentration of soybean oil is 5g / L,

[0120] The final concentration of calcium carbonate is 5g / L,

[0121] The pH value of the fermentation medium before digestion was 7.0.

[0122] Make the growth curve of S. spinosa. The growth curve of S. spinosa figure 2 shown. ...

Embodiment 2

[0130] (1) fermentation

[0131] Shanghai Guoqiang FUS-50L automatic fermenter system was used for fermentation, the proportion of the medium was the same as that of Example 1, and the control process was as follows:

[0132] Table 1

[0133] Control parameters

control point

quantity

35L

temperature

28℃±0.5℃

Rotating speed

Starting at 400rpm, adjusted according to dissolved oxygen

Ventilation ratio

1∶1

tank pressure

0.05MPa

dissolved oxygen

The whole process> 40%

cycle

10 days

[0134] (2) extract spinosad

[0135] Solid-phase extraction method: take the following method to extract spinosad from the fermentation broth (solid-phase extraction method): take 1 mL of fermentation broth and carry out solid-liquid separation, discard the liquid, and obtain the spinosad-containing mycelium in a solid state , after drying the above-mentioned mycelium, 0.4 g of mycelium was obt...

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Abstract

The invention relates to saccharopolyspora spinosa strain, application of the saccharopolyspora spinosa strain and a method for preparing spinosad. The strain related to the invention has the preservation number of CGMCC No.3460. The invention also relates to a mutant strain obtained by the strain. The invention also relates to the method for preparing the spinosad, which comprises the following steps of: a, fermenting the saccharopolyspora spinosa strain or the mutant strain thereof disclosed by the invention to obtain fermentation liquor containing the spinosad; and b, separating the spinosad from the fermentation liquor. The strain disclosed by the invention has strong capability of producing the spinosad and has high industrial application value.

Description

technical field [0001] The invention relates to a strain of Saccharopolyspora spinosa, its use and a method for preparing spinosad. Background technique [0002] Spinosad is a new type of microbial-derived insecticide with contact and ingestion toxicity. It has the excellent characteristics of broad spectrum and high efficiency against pests, extremely safe for humans, non-target animals and the environment, and biodegradable. Spinosad has a new mechanism of action, it does not act on acetylcholinesterase and Na + Channels, which are different from traditional organophosphate and pyrethroid insecticides. Experiments have proved that it has rapid contact and ingestion toxicity to insects. It works by stimulating the insect's nervous system, causing non-functional muscle contraction, failure, accompanied by tremors and paralysis. This action result is consistent with the activation of nicotinic acetylcholine receptors, and it is obvious that this action mechanism is novel a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 蒋勤军
Owner 牡丹江佰佳信生物科技有限公司
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