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Method for preparing a processed virtual analysis plate

A technology for analyzing plates and samples, applied in the field of cytological analysis, which can solve problems such as low correlation, poor cell classification, and difficult to deal with fuzzy areas

Inactive Publication Date: 2012-03-07
诺瓦西特公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Additionally, segmentation tools have a harder time dealing with ambiguous regions affecting isolated cells and 3D clusters
As a result, the analysis of cells and nuclei becomes less relevant and can even lead to poor cell classification

Method used

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  • Method for preparing a processed virtual analysis plate
  • Method for preparing a processed virtual analysis plate
  • Method for preparing a processed virtual analysis plate

Examples

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Embodiment Construction

[0042] see figure 1 , describes a method of preparing a virtual analysis panel 2 by viewing the analysis of cells assisted by a computer system. By virtual board 2 is meant a set of information and numerical data related to sample 4 .

[0043] For example, samples are obtained by scraping (cervical, vaginal or other swabbing), by puncturing an organ (breast, thyroid, ganglion or other) or further by collection (urine, tracheoalveolar lavage or other bodily fluids) 4 . During the first step A, the samples are temporarily stored, for example, in sampling tubes or flasks 6 .

[0044] During step B, the sample 4 is positioned on the analysis plate 8 . Deposition of the cells on the plate 8 is accomplished in a known manner, for example by decantation. The sample is poured into a decanting chamber 10 whose bottom opens into the analysis plate 8 . The absorption means 12 allow the gradual absorption of the solution as the cells are deposited onto the assay plate 8 . Such deposi...

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Abstract

The invention relates to a method that includes the following steps: carrying out a sample processing, the processing being carried out for differentiating diseased cells from healthy cells in the sample (4); carrying out at least one first image acquisition of the sample (4) provided on an analysis plate (8) so as to obtain a plurality of images, each representing an area (18) of the analysis plate, said images being arranged side by side so as to form an image of the entirety of the sample in order to create a virtual analysis plate (2); marking a reference plane of the analysis plate including a blade and a platelet provided above the blade for image acquisition, said reference plane being defined by the surface of the blade or the platelet; and carrying out at least one second image acquisition, said second acquisition being carried out at a different thickness of the sample relative to the first acquisition so as to obtain a plurality of images corresponding to a section of the sample with a different thickness.

Description

technical field [0001] The invention relates to a method for preparing a virtual analysis plate of a cytological sample positioned on an analysis plate in order to allow the cellular analysis of said sample, said type of method comprising the following steps: [0002] - performing a processing of the sample to distinguish diseased cells from healthy cells in the sample, [0003] - Performing image acquisition of the sample positioned on the analysis plate in order to obtain a plurality of images, each image representing a region of the analysis plate, said images being arranged side by side to form an image of the entire sample to generate a virtual analysis plate. [0004] The invention is particularly applicable to cytological analysis methods. Background technique [0005] Sample analysis e.g. for pathological diagnosis, by scraping (cervical, vaginal or other scraping), by puncture of an organ (chest, thyroid, ganglion or other) or further by collection (urine, tracheoa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/36
CPCG01N1/312G02B21/367
Inventor E·佩尔蒂埃
Owner 诺瓦西特公司
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