Application of silkie melanin extract to preparation of medicine for preventing and treating parkinson disease
A technology for Parkinson's disease and melanin is applied to the application field of silky chicken melanin extract in the preparation of medicines for preventing and treating Parkinson's disease, and achieving the effect of preventing and treating Parkinson's disease
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0045] Example 1. Black-bone fowl melanin extract in SHSY-5Y cell injury model induced by 6-OH DA Protective effects
[0046] 1. Experimental materials
[0047] 1. Black-bone fowl melanin extract: Take the silky fowl melanin extract prepared according to the above-mentioned method for extracting black-bone fowl melanin, and suspend it in normal saline at a concentration of 0.2g / ml.
[0048] 2), 10mM 6-OH DA storage solution: accurately weigh 5mg of 6-OH DA·HBr (purchased from SIGMA), dissolve it in 2ml of distilled water containing 0.01% vitamin C, filter with 0.22μm microporous membrane Sterilize and store in aliquots at -20°C. Dilute it to the appropriate concentration before use.
[0049] 3) SHSY-5Y cells: purchased from ATCC.
[0050] 4) SHSY-5Y cell culture medium: DMEM culture medium (purchased from SIGMA), adding 10% FBS (purchased from SIGMA).
[0051] 5) 0.125% trypsin-0.01% EDTA (pH 7.4, 1L): Take 12.5g trypsin (purchased from SIGMA) and 0.1g EDTA in the container, and then...
Embodiment 2
[0070] Example 2: The black-bone fowl melanin extract was used in MPP + Protective action in induced MN9D cell injury model use
[0071] 1. Experimental materials
[0072] 1) Silky fowl melanin extract: Take the finely-extracted silky fowl melanin prepared according to the above-mentioned method for extracting black-bone fowl melanin, and suspend it in normal saline at a concentration of 0.2g / ml for use.
[0073] 2), 20mM MPP + Storage solution: accurately weigh MPP + (Purchased from SIGMA) 100mg, add 16.8ml 0.01M PBS, filter and sterilize with 0.22μm microporous membrane, and store in aliquots at -20°C. Dilute to the corresponding concentration before use.
[0074] 3) MN9D cells: purchased from ATCC.
[0075] 4) MN9D cell culture medium: DMEM / F12 (purchased from Gibco), adding 10% NCS (calf serum, purchased from Gibco).
[0076] 5) 0.125% trypsin-0.01% EDTA (pH 7.4, 1L): Take 12.5g trypsin (purchased from SIGMA) and 0.1g EDTA in a container, and use 0.01M PBS (phosphate buffered s...
Embodiment 3
[0092] Example 3 Protective action of silky fowl melanin extract in MPTP-induced Parkinson's disease mouse model use
[0093] 1. Experimental materials
[0094] 1) Silky fowl melanin extract: Take the silky fowl melanin extract prepared according to the above-mentioned silky fowl melanin extraction method, and suspend it in normal saline at a concentration of 0.2g / ml for use.
[0095] 2). Healthy C57BL / 6 male mice: weighing 22g to 27g, provided by Beijing Weitonglihua Animal Breeding Center. Each cage is raised in groups of 4 to 5, the bottom of the cage is covered with sawdust, 12 hours / 12 hours day and night cycle lighting, free drinking and eating. Adapt to the environment for 4 to 5 days before the start of the experiment.
[0096] 3). Preparation of MPTP-HCL preparation used in the mouse model of Parkinson's disease: Dissolve 100mg of MPTP-HCL (purchased from SIGMA) in 20ml of normal saline, store in the dark at -80℃, and dilute to the required amount before use concentratio...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More