Method for simultaneously measuring content of three antitubercular agents in blood and tissues

An anti-tuberculosis and blood technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long test report time, increase the difficulty of clinical test, and large sample size requirements, and achieve short processing time and simple method , short time-consuming effect

CN102384949AInactive Publication Date: 2012-03-21蒋晖 +2
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2012-03-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for simultaneously measuring content of three antitubercular agents (retozide, rifampicin and pyrazinamide) in blood and tissues. The method has the characteristics of simplicity and convenience in operation, high precision, high detection speed, stability and reliability. A high-efficient liquid-phase chromatographic instrument (LC2010C), a column temperature control box AT-130 and an Lc-solution spectrum work station of the Japan Shimadzu Company are adopted. The chromatographic column is an analysis column and adopts a C8 column, the protection column adopts a C18 column, and the fluid phase adopts sodium 1-heptanesulfonate solution to prepare.
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Description

[0001] Field:

[0002] The invention belongs to a method for simultaneously measuring the contents of three anti-tuberculosis drugs (isoniazid, rifampicin, and pyrazinamide) in blood, and specifically relates to a method for simultaneously measuring three kinds of anti-tuberculosis drugs in blood and tissues by high-pressure liquid chromatography. A method for the content of anti-tuberculosis drugs. Background technique:

[0003] Isoniazid, Rifampicin, and Pyrazinamide are still the first-line anti-tuberculosis drugs widely used today. Isoniazid has neurotoxicity, and rifampicin has liver toxicity, so the concentration of anti-tuberculosis drugs Monitoring has very important clinical significance. The physical and chemical properties of these three drugs are completely opposite. Isoniazid and pyrazinamide are highly polar substances with high water solubility, while rifampicin is a weak polar substance with high fat solubility. It is difficult to completely separate the thre...

Examples

Embodiment 1

[0025] 1.1 The test sample is blood

[0026] 1.2 Chromatographic conditions:

[0027] The chromatographic column is the analytical column shim-pack HRC-C8 (250mm×4.6mm, 5μm), the guard column Eclipse XDB-C18 (125mm×4.6mm), the column temperature is 25C°; the mobile phase is 0.02mol / L, heptanesulfonic acid Sodium solution (dilute phosphoric acid to adjust the pH to 3.0) - acetonitrile - methanol (78:5:17) 6min, (20:5:75) 16min, (78:5:17) 27min. The flow rate is 0.8ml / min, the test wavelength Pyrazinamide: 268nm, isoniazid: 264nm, rifampicin: 254nm. Retention time respectively pyrazinamide: 5.62min, isoniazid: 10.01min, rifampicin: 24.89min.

[0028] 1.3 Chromatographic analysis results:

[0029] The chromatographic separation was good, and the three drugs did not interfere with each other with endogenous magazines in the blood. Figure 1 is Spectrum after blank plasma injection, Figure 2 is Chromatograms of blank plasma injected with three drugs, Figure 3 is The chromatog...