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Microbe and farm chemical composite bactericide and preparation method thereof

A technology of microbial pesticides and compound fungicides, applied in the directions of botanical equipment and methods, fungicides, biocides, etc., can solve the problems of affecting the habitat of crops and enhancing the drug resistance of bacteria, and achieves simple preparation method and production cost. Low, delayed enhancement effect

Inactive Publication Date: 2012-04-04
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of high doses not only affects the habitat of crops, but also enhances the resistance of pathogens

Method used

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  • Microbe and farm chemical composite bactericide and preparation method thereof
  • Microbe and farm chemical composite bactericide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] (1) Inoculate the Bacillus subtilis BAB-1 strain (preservation number: CGMCCNo.2099) into NB liquid medium. After 12 hours of cultivation, the strain reaches the logarithmic growth phase, and transfer it to 50mL of In PB liquid medium (contain beef extract 5g, NaCl 5g, peptone 10g, 1000mL water, pH 7.0-7.2 per liter), after 38 hours of cultivation, 95% of the bacteria formed spores, and the fermentation liquid of Bacillus subtilis was obtained. Add β-cyclodextrin with 10% of the mass of the fermentation liquid to the above fermentation liquid, and spray dry to obtain the original powder of Bacillus subtilis, the number of live bacteria in the original powder is 15-20 billion / g;

[0076] (2) Add mancozeb according to the mass ratio of the original powder: mancozeb=95:5, and mix to obtain effective active ingredients;

[0077] (3) Weigh each component according to the following parts by weight, and mix evenly to obtain a mixed powder:

[0078] 10 active ingredients

[0...

Embodiment 2

[0084] (1) Inoculate the Bacillus subtilis BS-03 strain (preservation number: CGMCCNo.3038) into the NB medium. After 12 hours of cultivation, the strain reaches the logarithmic growth phase, and transfer it to 50 mL of PB with a 5% inoculation amount. In the liquid culture medium (containing beef extract 5g, NaCl 5g, peptone 10g, 1000mL water, pH7.0-7.2 per liter), after 38 hours of cultivation, 95% of the bacteria formed spores to obtain the fermentation liquid of Bacillus subtilis. Add β-cyclodextrin with 10% of the mass of the fermentation broth to the fermentation broth, and spray dry to obtain the original powder of Bacillus subtilis, the number of live bacteria in the original powder is 15-20 billion / g;

[0085] (2) Add mancozeb according to the mass ratio of the original powder: mancozeb=99:1, and mix to obtain effective active ingredients;

[0086] (3) Weigh each component according to the following parts by weight, and mix evenly to obtain a mixed powder:

[0087] A...

Embodiment 3

[0093] (1) Inoculate the Bacillus subtilis sf-628 strain (preservation number: CGMCCNo.1772) into the NB medium. After 12 hours of cultivation, the strain reaches the logarithmic growth phase, and transfer it to 50 mL of PB with a 5% inoculation amount. In the liquid culture medium (containing beef extract 5g, NaCl 5g, peptone 10g, 1000mL water, pH7.0-7.2 per liter), after 38 hours of cultivation, 95% of the bacteria formed spores to obtain the fermentation liquid of Bacillus subtilis. Add β-cyclodextrin with 10% of the mass of the fermentation broth to the fermentation broth, and spray dry to obtain the original powder of Bacillus subtilis, the number of live bacteria in the original powder is 15-20 billion / g;

[0094] (2) Add mancozeb according to the mass ratio of the original powder: mancozeb=97:3, and mix to obtain effective active ingredients;

[0095] (3) Weigh each component according to the following parts by weight, and mix evenly to obtain a mixed powder:

[0096] ...

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Abstract

The invention relates to bacillus subtilis and mancozeb farm chemical composite bactericide, and a formula of a wettable powder formula and a preparation method thereof. The active components of the bactericide comprise 95 to 99 percent of bacillus subtilis original powder and 1 to 5 percent of mancozeb. The wettable powder can be prepared by adding 1 to 10 parts of wetting agent, 40 to 50 parts of filling material and 1 to 10 parts of carrier into the active components; and grinding in a fluidized bed air flow grinder to more than 200 meshes. In the composite bactericide, the synergistic effects of the bacillus subtilis and mancozeb are obvious, the pollution of the farm chemical to the environment and the residual of the farm chemical in fruits are reduced, borrytis cinerea and the like can be prevented and controlled effectively, and the increase in the medicine resistance in pathogenic bacteria is delayed. The preparation method of the bactericide disclosed by the invention is simple, and the production cost is low.

Description

technical field [0001] The present invention relates to a microbial pesticide compound fungicide, especially a fungicide compounded by Bacillus subtilis and mancozeb pesticide, and also relates to a wettable powder compounded by Bacillus subtilis and mancozeb pesticide and its Preparation. technical background [0002] In current agricultural production, due to the excessive use of chemical pesticides, the agricultural ecological environment is deteriorating day by day; the efficiency of agricultural production is low; the ecological environment is seriously polluted and the resistance of plant diseases is enhanced; it also has an impact on human health. However, biopesticides have the advantages of less pollution to the environment; they are easily decomposed and destroyed by other organisms or natural factors; they are not easy to accumulate in the environment. Biopesticides mainly include biopesticides, fungicides and ecological pesticides. Among them, bacterial fungici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N47/14A01N25/14A01P3/00
Inventor 张丽萍张根伟尹淑丽程辉彩黄亚丽周竞李书生
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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