Novel infectious disease nucleic acid rapid detection kit and its detection method

A technology for detecting kits and infectious diseases, which is applied in the field of rapid detection kits for nucleic acids of new infectious diseases, which can solve the problems of severe impact of sexually transmitted diseases detection, and achieve the effect of short operation time, simple operation and low cost

Inactive Publication Date: 2012-04-11
USTAR BIOTECHHANGZHOULTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the false positives of PCR have a greater impact on STD detection, and

Method used

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  • Novel infectious disease nucleic acid rapid detection kit and its detection method
  • Novel infectious disease nucleic acid rapid detection kit and its detection method
  • Novel infectious disease nucleic acid rapid detection kit and its detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Embodiment 1: the detection of sexually transmitted disease chlamydia infection (CT) sample

[0105] 1. Instrument-free nucleic acid extraction

[0106] 11 Elution: Take the cotton swab sample of the patient to be tested for CT, add 1ml of normal saline to the sample collection tube, shake and mix well to ensure that the secretion on the cotton swab is fully eluted;

[0107] 1.2 Cleavage: Add an equal amount of lysate to the above eluent; mix well and let it stand at room temperature for 5 minutes; use a micro-clinical sample nucleic acid rapid extraction device to suck up the above mixed solution, push out the filtrate in the suction device and discard;

[0108] 1.3 Cleaning: Use the micro-clinical sample nucleic acid rapid extraction device to suck in the centrifuge tube containing the cleaning solution, then push out the filtrate in the suction device and discard it;

[0109] 1.4 DNA elution: continue to use the micro-clinical sample nucleic acid rapid extraction de...

Embodiment 2

[0123] Example 2 Detection of sexually transmitted disease Ureaplasma urealyticum (UU) sample

[0124] 1. Instrument-free nucleic acid extraction

[0125] 1.1 Elution: Take the cotton swab sample of the UU patient to be tested, add 1ml of normal saline to the sample collection tube, shake and mix well to ensure that the secretions on the cotton swab are fully eluted;

[0126] 1.2 Cleavage: add an equal amount of lysate to the eluent above; mix well and let it stand at room temperature for 5 minutes; use a micro-clinical sample nucleic acid rapid extraction device to suck up the above mixture, push out the filtrate in the suction device and discard it;

[0127] 1.3 Cleaning: Use the micro-clinical sample nucleic acid rapid extraction device to suck in the centrifuge tube containing the cleaning solution, then push out the filtrate in the suction device and discard it;

[0128] 1.4 DNA elution: Continue to use the micro-clinical sample nucleic acid rapid extraction device to su...

Embodiment 3

[0142] The detection of embodiment 3 sexually transmitted disease Neisseria gonorrhoeae (NG) sample:

[0143] 1. Instrument-free nucleic acid extraction

[0144] 1.1 Elution: Take a cotton swab sample from a patient to be tested for NG, add 1ml of normal saline to the sample collection tube, shake and mix well to ensure that the secretions on the cotton swab are fully eluted;

[0145] 1.2 Cleavage: add an equal amount of lysate to the eluent above; mix well and let it stand at room temperature for 5 minutes; use a micro-clinical sample nucleic acid rapid extraction device to suck up the above mixture, push out the filtrate in the suction device and discard it;

[0146] 1.3 Cleaning: Use the micro-clinical sample nucleic acid rapid extraction device to suck in the centrifuge tube containing the cleaning solution, then push out the filtrate in the suction device and discard it;

[0147] 1.4 DNA elution: Continue to use the micro-clinical sample nucleic acid rapid extraction dev...

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Abstract

The invention relates to a novel infectious disease nucleic acid rapid detection kit and its detection method, the rapid detection kit comprises an apparatus-free micro nucleic acid extraction kit, a nucleic acid constant temperature amplification reaction kit and a nucleic acid anti-pollution detection apparatus. The apparatus-free micro nucleic acid extraction kit comprises a micro nucleic acid extraction apparatus, a suction device, a cell lysate, a cleaning solution and an eluate. The rapid detection kit has the advantage that the apparatus-free nucleic acid extraction is carried out. The detection kit has the advantages of strong specificity, high sensitivity, simple operation, rapidity and no apparatus requirement during the detection process.

Description

technical field [0001] The invention relates to a kit for detecting nucleic acid, in particular to a kit for rapid detection of nucleic acid for novel infectious diseases and a detection method thereof. Background technique [0002] Infectious diseases are globally transmitted diseases, and the incidence rate is increasing year by year. At present, in the experimental diagnosis of infectious disease infection, cell culture method can be used, which has strong specificity, but it is time-consuming, expensive, and not sensitive enough, so it is not suitable for clinical development; immunological methods are simple and fast, and have practicality in primary hospitals ; In hospitals with molecular biology experimental conditions, because the polymerase chain reaction (Polymerase Chain Reaction, PCR) method is highly sensitive and specific, it can detect people with various incidence rates. As long as the operation is strictly standardized and effective measures are taken, false...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 胡林祁军尤其敏李波石坚马玉霞
Owner USTAR BIOTECHHANGZHOULTD
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