Plane parallel fluorescent biological detecting chip based on total reflection and preparation method thereof

Abstract

The invention discloses a plane parallel fluorescent biological detecting chip based on total reflection and a preparation method thereof. In the chip, the sample table is bonded by glass sheets and PDMS chips and located in the top surface of the whole chip; a prism, a lens, an optical fiber stopping part, an optical fiber supporting part and optical fiber that are on the same level are arranged below the sample table; the prism, the lens, the optical fiber stopping part and the optical fiber supporting part are all made from PDM integrally; when laser comes into the single-mode fiber after being coupled, the light beam transmits in the optical fiber; the emergent light is corrected by the lens and is reflected when the emergent light reaches the air / prism interface; then the reflected light reaches the prism / glass interface and is further reflected; the reflected light is fully reflected on the glass / water interface when the reflected light comes into glass; and evanescent wave is generated in the full-reflecting zone for generating fluorescence reaction of the sample in water. The chip is easy to be integrated with mini-analyzing system without laser source. Therefore, the detecting chip is low in biological material consumption, low fluorescent background noise and high sensitivity.

Description

technical field [0001] The invention relates to a chip structure for fluorescent biological detection, in particular to a planar parallel fluorescent biological detection chip based on total reflection and a preparation method thereof. Background technique [0002] In recent years, total reflection fluorescence microscopy has received extensive attention because it is suitable for the study of molecular dynamics near the surface at the single-molecule level. The working principle of total reflection fluorescence microscopy relies on evanescent waves generated by total reflection. Compared with traditional fluorescence microscopy, the use of evanescent waves to excite fluorophores can reduce the background noise to a minimum, thus making the microscopic effect clearer. [0003] After searching the literature of the prior art, it was found that "TIR Fluorescence Reader for Selective Detection of Cell Membranes" published by Thomas Bruns in 2005 (a total reflection fluorescenc...

AI Technical Summary

Problems solved by technology

The large size of this kind of reader is not conducive to integration, and the consumption of biological materials is large. It needs to be aligned every time it is used. When observing with a CCD camera, a filter device is required to reduce background noise

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