Achromobacter xylosoxidans and application thereof to degradation of isoquinoline

A colorless bacillus, isoquinoline technology, applied in bacteria, chemical instruments and methods, microorganism-based methods, etc., can solve the problems of unoptimistic status of large-scale regeneration treatment of coking wastewater

Inactive Publication Date: 2012-04-18
WUHAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on coking wastewater has made great progress, but the current situation of large-scale regeneration and treatment of coking wastewater is still not optimistic. The technology

Method used

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  • Achromobacter xylosoxidans and application thereof to degradation of isoquinoline
  • Achromobacter xylosoxidans and application thereof to degradation of isoquinoline
  • Achromobacter xylosoxidans and application thereof to degradation of isoquinoline

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Effect test

Embodiment 1

[0015] Isolation, Purification and Identification of Achromobacter xylosoxidans Y-4

[0016] 1. Isolation and purification of Achromobacter xylosoxidans Y-4

[0017] 1) Sample collection

[0018] Take the activated sludge from the coking wastewater treatment system of a coking plant for pretreatment, that is, add an appropriate amount of sterile water and stir in a constant temperature magnetic stirrer for 30 minutes to increase the activity of bacteria in the activated sludge.

[0019] 2) Enrichment culture and domestication

[0020] Add 2 to 5 glass beads in turn to the conical flask containing 100mL of coking wastewater, the 100mL conical flask of MSM liquid medium containing 50% coking wastewater, and the 250mL conical flask of 100mL MSM liquid medium. , two drops of 0.01% sodium pyrophosphate solution, 0.02 g of isoquinoline and 5 mL of treated activated sludge, cultivated in a constant temperature shaker at 30 ° C and 150 r / min for 2 to 4 days to prepare three culture ...

Embodiment 2

[0036]Degradation effect of Achromobacter xylosoxidans Y-4CCTCC NO: M2011308 on isoquinoline

[0037] Achromobacter xylosoxidans (Achromobacter xylosoxidans) Y-4 was expanded and cultured in LB liquid medium, then centrifuged at 6000r / min for 5min, the supernatant was discarded, and an appropriate amount of sterilized MSM liquid medium was added to resuspend. Then centrifuge at 6000r / min for 5min, discard the supernatant, and then add an appropriate amount of sterilized MSM liquid medium to resuspend to make OD 600 1 bacterial suspension for inoculation.

[0038] 1) The prepared OD 600 The bacterial suspension of 1 was inoculated into the sterilized MSM liquid medium containing isoquinoline with a concentration of 500mg / L, and cultivated in a constant temperature shaker at 150r / min. The temperature of the constant temperature shaker was set at 10°C, 20°C, °C, 30 °C and 40 °C, the degradation rate is measured by GC after regular sampling and extraction, and the degradation ef...

Embodiment 3

[0044] Application of Achromobacter xylosoxidans Y-4CCTCC NO: M2011308 Loaded on Activated Carbon for Biological Purification of Isoquinoline in Coking Wastewater

[0045] 1) Achromobacter xylosoxidans Y-4 is expanded and cultured with LB and inorganic salt liquid medium to obtain cultured Achromobacter xylosoxidans Y-4 bacterial liquid.

[0046] 2) Load the cultivated Achromobacter xylosoxidans Y-4 bacterial solution on the activated carbon carrier by intermittently feeding water, and at the same time, pass air into it for continuous aeration, and hang the film for 1 to 2 weeks. The local area of ​​the carrier forms a thin biofilm, and finally forms a layer of mature biofilm that completely wraps the carrier, that is, the Achromobacter xylosoxidans Y-4 strain is successfully loaded on the activated carbon to obtain biological activated carbon.

[0047] 3) MSM liquid medium containing isoquinoline and COD of 150-200 was passed into the biological activated carbon, treated by c...

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Abstract

The invention discloses Achromobacter xylosoxidans and application thereof to degradation of isoquinoline. The conservation registration number of the Achromobacter xylosoxidans strain which is named Y-4 is CCTCC No. M2011308, and the Genbank accession number of the strain 16SrDNA is JN381516. The Achromobacter xylosoxidans Y-4 has unique degradationeffect on the isoquinoline, the maximum tolerance concentration (MTC) for the isoquinoline can reach 1000mg/L, and the isoquinoline with the concentration of not more than 500mg/L can be fully degraded in 36 hours, the optimal degradation temperature is 25-35 DEG C, the pH value is 4-8; meanwhile the Achromobacter xylosoxidans Y-4 also has better degradation effect on naphthalene, pyridine and quinoline, and has obvious effect particularly for the isoquinoline in biological purified coking wastewater. According to the Achromobacter xylosoxidans and application thereof disclosed by the invention, an important basis is provided for research on micro-biological degradation of the isoquinoline in the coking wastewater, and an important role can be played in practice of quinoline industrial wastewater treatment.

Description

technical field [0001] The invention belongs to the technical field of Achromobacter xylosoxidans. It specifically relates to a strain of Achromobacter xylosoxidans and its application in degrading isoquinoline. Background technique [0002] Due to the high toxicity of coking wastewater and the high content of refractory organic matter, it is difficult for the COD concentration and chromaticity of the effluent after conventional biochemical treatment to meet the national recycling water standards. After the A-A-O process, its chromaticity and COD value did not reach the standard of reclaimed water, and 118 kinds of organic compounds were still detected by GC-MS. Among them, the content of quinoline organic matter is the highest, accounting for 14.3wt% of the total organic matter content. That is to say, quinoline organic compounds are colored polycyclic aromatic hydrocarbons that are difficult to degrade in the coking wastewater treatment system, and their content has dire...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/025C02F101/38
Inventor 王光华杨书琴蒋丽娟郭胜李文兵刘文敏刘奇
Owner WUHAN UNIV OF SCI & TECH
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