Cryogenic storing protection liquid for human umbilical cord Wharton's jelly tissue block

Abstract

The invention discloses a cryogenic storing protection liquid for a human umbilical cord Wharton's jelly tissue block. One liter of cryogenic storing protection liquid comprises 1-1.4 mol of permeable cryoprotectant, 0.1-0.5 mol of impermeable cryoprotectant and the balance of base liquid. The cryogenic storing protection liquid provided by the invention contains the permeable cryoprotectant and the impermeable cryoprotectant at the same time, the concentration of the permeable cryoprotectant is low, so that good cryogenic storage effect can be achieved, and the toxicity of cells is also small. Meanwhile, the cryogenic storing protection liquid contains the base liquid, not only can nutrients be provided for the human umbilical cord Wharton's jelly tissue block, but also the toxicity of cryoprotectants on cells in the Wharton's jelly tissue block is lowered. The human umbilical cord Wharton's jelly tissue block cryogenically stored by the cryogenic storing protection liquid can be used for preparing high quality umbilical cord mesenchymal stem cells, few cells are damaged, and the cryogenic storing protection liquid has small residue, small toxic and side effects and no harm to human bodies in clinic use.

Description

technical field [0001] The invention relates to a cryopreservation solution for human umbilical cord Wharton's jelly tissue blocks. Background technique [0002] The cells in human umbilical cord Wharton's Jelly (Wharton's Jelly) have the characteristics of stem cells such as self-renewal, multi-directional differentiation, and strong proliferation ability. We call them umbilical cord mesenchymal stem cells (huMSCs). Mesenchymal stem cells are an important member of the stem cell family. They are low-immunogenic stem cells. They have the characteristics of supporting hematopoiesis, promoting stem cell implantation, and regulating immunity. They are ideal seeds for repairing tissue and organ damage caused by aging and disease. cell. Umbilical cord mesenchymal stem cells are getting more and more attention because of their wide source, easy access, and all the characteristics of mesenchymal stem cells. More and more people start to store umbilical cord mesenchymal stem cells ...

AI Technical Summary

Problems solved by technology

Under normal circumstances, people choose a single permeable cryoprotectant dimethyl sulfoxide to cryopreserve stem cells. After cryopreservation and recovery, there will be residual cryoprotectant solution. Clinically, stem cells with residual cryoprotectant dimethyl sulfoxide are used. , has high toxic and side effects, and can easily cause nausea, vomiting, abdominal pain and other adverse reactions

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