Determination method for ammonia (ammonia ions) and ammonia (ammonia ion) diagnostic/assay kit
A technology of ammonia ions and reagents, which is applied in the field of medical/food inspection and determination, and can solve problems such as undiscovered blood ammonia determination methods
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Embodiment 1
[0126] Embodiment 1: the mensuration of ammonia (ammonia ion) content in blood plasma
[0127] 1) Preparation of standard samples
[0128] Dissolve a certain amount of ammonia salt in water or buffer solution, and then adjust the ammonia concentration to 100 micromole / liter;
[0129] 2) Pretreatment of samples to be tested
[0130] Plasma samples are used as samples to be tested without pretreatment;
[0131] 3) Blank sample
[0132] Described water or buffer solution are used as blank sample, and its ammonia concentration is 0 micromole / liter;
[0133] B. Preparation of reagent solution:
[0134] The ammonia diagnosis / measurement reagent of the present embodiment is a single-dose reagent, which contains:
[0135] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0136] Glycerin 1mol / L
[0137] NADPH0.25mmol / L
[0138] Ammonia kinase 16000U / L
[0139] Pyruvate kinase 18000U / L
[0140] N-methylalanine dehydrogenase 12000U / L
[0141] Adenosine triph...
Embodiment 2
[0157] Embodiment 2: the mensuration of ammonia (ammonia ion) content in blood plasma
[0158] 1) Preparation of standard samples
[0159] A certain amount of ammonia salt is dissolved in water, and then the concentration of ammonia (ammonia ion) is adjusted to 100 micromole / liter, as a standard sample;
[0160] 2) Preparation of samples to be tested
[0161] Plasma samples are used as samples to be tested without pretreatment;
[0162] 3) Blank sample
[0163] Described water is used as blank sample, and its ammonia (ammonia ion) concentration is 0 micromole / liter;
[0164] B. Preparation of reagent solution:
[0165] Ammonia (ammonia ion) diagnostic / determination reagent is a two-dose reagent, which contains:
[0166] Reagent 1 with the following composition:
[0167] Phosphate buffer 100mmol / L
[0168] NADH0.25mmol / L
[0169] Adenosine triphosphate 5mmol / L
[0170] Enol pyruvate 5mmol / L
[0171] Methylamine 5mmol / L;
[0172] Reagent 2 with the following compositi...
Embodiment 3
[0194] Embodiment 3: the mensuration of ammonia (ammonia ion) content in plasma sample
[0195]The mode of operation of this embodiment is the same as embodiment 2, but this embodiment:
[0196] B. Preparation of reagent solution:
[0197] Ammonia (ammonia ion) diagnostic / assay reagent is a three-dose reagent that contains:
[0198] Reagent 1 with the following composition:
[0199] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0200] NADH0.25mmol / L
[0201] Adenosine triphosphate 6mmol / L
[0202] Enol pyruvate 5mmol / L
[0203] Methylamine 4mmol / L;
[0204] Reagent 2 with the following composition:
[0205] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0206] Sodium chloride 2mol / L
[0207] Pyruvate kinase 28000U / L
[0208] N-methylalanine dehydrogenase 50000U / L;
[0209] Reagent 3 with the following composition:
[0210] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
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