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Method for determining content of eugenol in formulated cut tobacco

A technology of eugenol and formula shredded tobacco, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve high sensitivity, good linear correlation, and good repeatability

Active Publication Date: 2012-06-13
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no regulations in this regard in the country

Method used

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  • Method for determining content of eugenol in formulated cut tobacco
  • Method for determining content of eugenol in formulated cut tobacco
  • Method for determining content of eugenol in formulated cut tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The present embodiment is as follows to the detection method of eugenol content in shredded tobacco (the flow chart of described detection method is as follows figure 1 shown)

[0034] (1) Preparation of extractant containing internal standard: absolute ethanol solution containing internal standard (anethole) at appropriate concentration, generally at a concentration of 0.1mg / mL to 0.2mg / mL;

[0035] (2) Preparation of standard solution: Weigh about 0.2g of eugenol, accurate to 0.0001g, dissolve it with the extraction agent containing the internal standard, transfer it to a 100mL volumetric flask, and dilute to volume as the standard stock solution;

[0036] Pipette 0.01mL, 0.1mL, 0.5mL, 1.0mL, 2.5mL, 5.0mL standard stock solutions into 10mL volumetric flasks, and use extractant to make up the volume, then the concentration of eugenol is 2.0μg / mL, 20μg / mL mL, 100μg / mL, 200μg / mL, 500μg / mL, 1000μg / mL; store the standard solution in the dark at 4°C, place it at room tempe...

Embodiment 2

[0053] The present embodiment is as follows to the detection method of the repeatability of the inventive method and the standard addition recovery rate

[0054] Using the sample addition recovery rate test, add 20.8μg / g, 208.0μg / g, and 1040.0μg / g standard solutions of three different concentrations in the sample to carry out the standard addition recovery rate test, each sample is measured 6 times respectively, and the chromatographic The condition of analysis is with embodiment 1, according to analysis result, calculate the recovery rate of standard addition of eugenol in shredded tobacco of this method and the relative standard deviation of measured value after standard addition, the results are as shown in table 2;

[0055] Table 2 The recovery and repeatability of eugenol in shredded tobacco (n=6)

[0056]

[0057] As can be seen from Table 2, at the three spiked levels, the average recovery rate of eugenol in shredded tobacco using this method is 101.14%, and the aver...

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Abstract

The invention relates to a method for determining the content of eugenol in formulated cut tobacco. The method comprises the steps of: preparation of an extraction reagent and a sample solution, liquid chromatogram analysis and detection result calculation, etc. The optimized detection method has the advantages of short detection time, simple operation, high sensitivity, and good repeatability, etc. The chromatographic condition of the method in the invention makes the chromatographic peak of eugenol and the chromatographic peak of impurities well separated. And the method of the invention also has good linear range, the detection limit is 0.3 microgram / g, the average recovery rate of standard addition is 101.4%, and the average relative standard deviation is less than 2.4%.

Description

[0001] technical field [0002] The invention relates to an improved method for determining the content of eugenol in formula shredded tobacco, belonging to the technical field of physical and chemical inspection of tobacco raw materials. [0003] Background technique [0004] Eugenol, 4-allyl-2-methoxyphenol, colorless to light yellow liquid, turns brown in the air, has a strong smell of cloves. Very soluble in water, soluble in ethanol, ether, chloroform and essential oils. As a fragrance agent, it is used in the preparation of carnation-type essence and in the preparation of isoeugenol and vanillin, etc. Also used as an insecticide and antiseptic, antioxidant, carminative, and in dentistry as an analgesic. In the carcinogenic list published by the International Agency for Research on Cancer (IARC), eugenol is listed as Group 3. Krete cigarette is a unique cigarette in Indonesia and Malay. Its taste is fragrant, delicious, refreshing and refreshing. [0005] On Septem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 张洪非程倩刘楠边照阳唐纲岭陈再根侯宏卫姜兴益李中皓李雪陈欢庞永强胡清源
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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