Thermal transmission detection method based on DNA (Deoxyribonucleic Acid) amplification

Abstract

The invention relates to a thermal transmission detection method based on DNA (Deoxyribonucleic Acid) amplification. In the detection method, a detection system is established on a DNA amplification module by using an optical fiber bragg grating sensor, the conventional platinum resistance sensor and a flow detection module; the detection system comprises an optical fiber bragg grating detection subsystem and a platinum resistance sensor detection subsystem; the optical fiber bragg grating detection subsystem comprises an optical fiber bragg grating sensor and a signal demodulating system; the optical fiber bragg grating sensor comprises a sensing probe; the signal demodulating system is connected with the sensing probe; the sensing probe is inserted into a reaction hole; the platinum resistance sensor detection subsystem comprises a control unit and a platinum resistance sensor; and the flow detection module is connected with the optical fiber bragg grating detection subsystem and the platinum resistance sensor detection subsystem through an interface module.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a heat transfer detection method based on DNA amplification. Background technique [0002] The steps of introducing DNA polymerase into PCR are as follows: the DNA reaction solution is placed in a test tube, the test tube containing the reaction solution is placed in each well of the PCR module in the reaction chamber, and the gene amplification process is automatically completed by the control program. So far, scholars at home and abroad have mainly used automatic control technology to control the temperature change of the PCR module in the control program. The Stephanie J. Culler research group used PCR equipment to analyze the internal structure expression of genes, but most of the research results focused on improving PCR. technology and improve certain indicators of PCR equipment. As early as 2001, E.T. Lagally used the method of increasing the heating and cooling r...

AI Technical Summary

Problems solved by technology

In the clinical application of PCR, a common problem is that the state of the DNA reaction solution in the test tubes of each well of the module changes with the different reaction stages. During this process, the chemical bonds of DNA are constantly broken or generated, accompanied by The state of heat absorption and heat release is also different. How to analyze the law of these state changes has a direct impact on improving the quality of DNA amplification and accurately controlling the temperature change of the test tube reaction solution placed in each well of the module. The operation of the prior art device is complicated. The safety and reliability of the system is low, the existing technology is not ideal for the detection effect, and the application in actual engineering has certain limitations

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