Novel heat transfer detection equipment

Abstract

The invention relates to novel heat transfer detection equipment. The equipment comprises a DNA reaction chamber, an optical fiber data processing module, a heat control processing module, and a main processor; the DNA reaction chamber is an enclosed space, a DNA amplification module is arranged in the DNA reaction chamber, and the upper part of the DNA amplification module is provided with a reaction hole; the reaction hole is internally provided with a Bragg sensing probe, the Bragg sensing probe is connected to an optical fiber core, the optical fiber core is connected to a demodulation module, the demodulation module is connected to the optical fiber data processing module, the optical fiber data processing module is connected to a comparator through a signal line, and the comparator is connected to the main processor through a signal line. Bragg gratings and a platinum resistance sensor are separately used for detection, centralized processing, construction of a data control model, simulation of a DNA whole amplification process, and disclosing of influence factors to a DNA sample; and a basis is provided for revealing objective rules of reactions in DNA.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a novel heat transfer detection device. Background technique [0002] A basic cyclic process of DNA gene amplification consists of three reaction stages: denaturation-annealing-extension. After dozens of basic cyclic processes, the gene to be amplified can be amplified by millions of times. In the three basic reaction stages, the temperature of the DNA denaturation stage is generally 93°C-95°C, and the double-stranded DNA template forms single-stranded DNA after the hydrogen bond is broken under the heat of this stage; the temperature of the annealing stage is generally 42°C -55°C, at this stage, the system temperature drops, and the primers combine with the DNA template to form a partial double strand; the temperature of the extension stage is generally 70°C-75°C, and at this stage, the DNA strand complementary to the DNA template is synthesized under th...

AI Technical Summary

Problems solved by technology

In the clinical application of PCR, a common problem is that the state of the DNA reaction solution in the test tubes of each well of the module changes with the different reaction stages. During this process, the chemical bonds of DNA are constantly broken or generated, accompanied by The state of heat absorption and heat release is also different. How to analyze the law of these state changes has a direct impact on improving the quality of DNA amplification and accurately controlling the temperature change of the test tube reaction solution placed in each well of the module. The operation of the prior art device is complicated. It cannot be flexibly and accurately detected according to the needs, and its application in actual engineering has certain limitations

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