DNA (Deoxyribonucleic Acid) amplification-based heat transfer detection device

Abstract

The invention relates to a DNA (Deoxyribonucleic Acid) amplification-based heat transfer detection device, which comprises a DNA reaction chamber, an optical fiber data processing module, a heat control processing module and a main processor. The DNA reaction chamber is a closed space; a DNA amplification module is arranged in the DNA reaction chamber; reaction holes are arranged in an upper partof the DNA amplification module; Bragg sensing probes are arranged in the reaction holes; the Bragg sensing probes are connected with optical fiber cores; the optical fiber cores are connected with ademodulation module; the demodulation module is connected with the optical fiber data processing module; the optical fiber data processing module is connected with a comparator through a signal wire;and the comparator is connected with the main processor through a signal wire. According to the DNA amplification-based heat transfer detection device, a fiber Bragg grating and platinum resistance sensors are adopted to respectively detect, the centralized processing is carried out, a data control module is constructed, the whole amplification process of DNA is simulated, the influencing factorson a DNA sample are disclosed, and a basis for disclosing the objective laws of the internal reaction of DNA is provided.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a heat transfer detection device based on DNA amplification. Background technique [0002] A basic cyclic process of DNA gene amplification consists of three reaction stages: denaturation-annealing-extension. After dozens of basic cyclic processes, the gene to be amplified can be amplified by millions of times. In the three basic reaction stages, the temperature of the DNA denaturation stage is generally 93°C-95°C, and the double-stranded DNA template forms single-stranded DNA after the hydrogen bond is broken under the heat of this stage; the temperature of the annealing stage is generally 42°C -55°C, at this stage, the system temperature drops, and the primers combine with the DNA template to form a partial double strand; the temperature of the extension stage is generally 70°C-75°C, and at this stage, the DNA strand complementary to the DNA template is...

AI Technical Summary

Problems solved by technology

In the clinical application of PCR, a common problem is that the state of the DNA reaction solution in the test tubes of each well of the module changes with the different reaction stages. During this process, the chemical bonds of DNA are constantly broken or generated, accompanied by The state of heat absorption and heat release is also different. How to analyze the law of these state changes has a direct impact on improving the quality of DNA amplification and accurately controlling the temperature change of the test tube reaction solution placed in each well of the module. The operation of the prior art device is complicated. It cannot be flexibly and accurately detected according to the needs, and its application in actual engineering has certain limitations

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