Novel heat conduction detection equipment

Abstract

The invention relates to a new type of heat transfer detection equipment, which includes a DNA reaction chamber, an optical fiber data processing module, a thermal control processing module and a main processor. Amplification module, a reaction hole is arranged on the upper part of the DNA amplification module, a Bragg sensing probe is arranged in the reaction hole, the Bragg sensing probe is connected with an optical fiber core, and the optical fiber core is connected with a demodulation module, the solution The tuning module is connected to the optical fiber data processing module, the optical fiber data processing module is connected to a comparator through a signal line, and the comparator is connected to the main processor through a signal line. The invention adopts fiber Bragg grating and platinum resistance sensor to detect separately, centralize processing, construct data control model, simulate the whole amplification process of DNA, reveal the influencing factors on DNA sample, and provide basis for revealing the objective law of DNA internal reaction.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a novel heat transfer detection device. Background technique [0002] A basic cyclic process of DNA gene amplification consists of three reaction stages: denaturation-annealing-extension. After dozens of basic cyclic processes, the gene to be amplified can be amplified by millions of times. In the three basic reaction stages, the temperature of the DNA denaturation stage is generally 93°C-95°C, and the double-stranded DNA template forms single-stranded DNA after the hydrogen bond is broken under the heat of this stage; the temperature of the annealing stage is generally 42°C -55°C, at this stage, the system temperature drops, and the primers combine with the DNA template to form a partial double strand; the temperature of the extension stage is generally 70°C-75°C, and at this stage, the DNA strand complementary to the DNA template is synthesized under th...

AI Technical Summary

Problems solved by technology

In the clinical application of PCR, a common problem is that the state of the DNA reaction solution in the test tubes of each well of the module changes with the different reaction stages. During this process, the chemical bonds of DNA are constantly broken or generated, accompanied by The state of heat absorption and heat release is also different. How to analyze the law of these state changes has a direct impact on improving the quality of DNA amplification and accurately controlling the temperature change of the test tube reaction solution placed in each well of the module. The operation of the prior art device is complicated. It cannot be flexibly and accurately detected according to the needs, and its application in actual engineering has certain limitations

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