Method for enriching vibrio phage and biologically preventing host bacteria

A technology of phage and host bacteria, which is applied in marine economic animal breeding sea areas and farms, can solve the problems of restricting phage application and poor operability, and achieve the effect of preventing vibriosis

Inactive Publication Date: 2013-07-24
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problem of using phage biological control at the "effective timing" or in advance makes the operability of phage biological control extremely poor, which greatly limits the application of phage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] shrimp farming pond

[0032] A film-type high-density shrimp farm with a winter shed was built in 430 mu of Donghai Island of Zhanjiang Tengfei Industrial Co., Ltd. In 2009 and 2010, the winter shed shrimp farming was completed. 30m built in 2009 2 , There are four 1.5m deep cement small pools, located in the four corners, add fresh filtered seawater (aquaculture water source water), and keep the water level not lower than 1m. Set up multi-layer filtration in the drainage channel, then hang the bag, and contain chlorine-containing disinfectant, and discharge it after 1-2 days of disinfection;

[0033] Four oysters grown in the same sea area were placed in each pool, about 10 oysters per hoist, and were soaked in fresh seawater for 48 hours before being placed. Control planktonic algae not higher than 1~5×10 by changing water, amino acid fertilizer, a small amount of fish meal and soybean meal 3 / mL, zooplankton not higher than 2×10 2 / mL, the Vibrio concentration s...

Embodiment 2

[0039] Plastic film shrimp pond culture Dongfeng snail

[0040] 25 shrimp ponds with 150 acres of 25 first-class water-lifting ponds in Donghai Island Anli were rebuilt as the Dongfeng snail pond site for plastic film shrimp ponds. Renovate the size of the pond, lay mulch film on the wall and bottom of the pond; renovate the water supply and drainage system, and add oxygen-enhancing facilities to make it a breeding system for Dongfeng snails. The mulch shrimp pond is laid with 5cm to 10cm of sand. Neither too fine sand nor too coarse sand can be used for screening with 10-mesh or 30-mesh sifter, and then the sand of the middle size is evenly laid on the bottom of the pool. The water depth is 60cm to 100cm. Equipped with 1 to 2 waterwheel type aerators with a power of 1.5kW;

[0041] 30m built in 2010 2 , 2 small cement pools with a depth of 1.5m, located near the drain, add fresh filtered seawater (aquaculture water source water), and keep the water level not lower than 1m...

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PUM

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Abstract

The invention relates to a method for enriching vibrio phage and biologically preventing host bacteria. Due to splitting specificity of the phage, the phage of the specific host strains in the specific environment and a certain state cannot be obtained easily. According to the facts that the vibrio strains and the phage of culture zones and sea areas coexist with the environment ecologically and evolutionally, a long-term culture pool of the host bacteria and the phage is built in a specific area, lytic phage of specific pathogenic bacteria hosts in the area can be continuously enriched and separated, the lytic phage can be placed in the area after augmentation, proliferation of pathogenic bacteria of a cultivation water body can be controlled in a short time, concentration of the pathogenic bacteria of the water body can be restrained in a long term, and the effect of ecological defense is achieved. The method utilizes the phage in the cultivation sea water body to prevent diseases caused by the pathogenic bacteria and has the advantages of being efficient, ecological, safe, safe and lasting.

Description

technical field [0001] The invention relates to a method for enriching Vibrio phage and biologically controlling host bacteria. The invention belongs to the microbial technology for disease prevention and control in seawater aquaculture, and is suitable for marine economic animal breeding sea areas and farms. Background technique [0002] Vibrio, as a common opportunistic pathogen in aquaculture, brings great harm every year. Opportunistic pathogens are also called opportunistic pathogens, and bacteria that can cause disease under certain conditions are called opportunistic pathogens. When the accumulation site of opportunistic pathogenic bacteria in the body changes, the body resistance is reduced or the flora is imbalanced, it can cause disease. The deterioration of water quality conditions in the water body, the increase of the concentration of opportunistic pathogens in the water body, and the decrease of the immune function of farmed animals can all lead to diseases. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00C12N1/20C12R1/63
CPCY02A40/81
Inventor 邱德全王成桂邱明生杨世平
Owner GUANGDONG OCEAN UNIVERSITY
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