Detection method for ammonia (ammonia ions), and ammonia (ammonia ion) diagnosis/detection kit
A technology of ammonia ions and reagents, which is applied in the field of medical/food inspection and determination, and can solve problems such as undiscovered blood ammonia determination methods
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Embodiment 1
[0131] Embodiment 1: the mensuration of ammonia (ammonia ion) content in blood plasma
[0132] 1) Preparation of standard samples
[0133] Dissolve a certain amount of ammonia salt in water or buffer solution, and then adjust the ammonia concentration to 100 micromole / liter;
[0134] 2) Pretreatment of samples to be tested
[0135] Plasma samples are used as samples to be tested without pretreatment;
[0136] 3) Blank sample
[0137] Described water or buffer solution are used as blank sample, and its ammonia concentration is 0 micromole / liter;
[0138] B. Preparation of reagent solution:
[0139] The ammonia diagnosis / measurement reagent of the present embodiment is a single-dose reagent, which contains:
[0140] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0141] Glycerin 1mol / L
[0142] NADPH0.25mmol / L
[0143] Ammonia kinase 16000U / L
[0144] Carbamyl phosphate synthase 18000U / L
[0145] Pyruvate dehydrogenase 12000U / L
[0146] Adenosine ...
Embodiment 2
[0162] Embodiment 2: the mensuration of ammonia (ammonia ion) content in blood plasma
[0163] 1) Preparation of standard samples
[0164] A certain amount of ammonia salt is dissolved in water, and then the concentration of ammonia (ammonia ion) is adjusted to 100 micromole / liter, as a standard sample;
[0165] 2) Preparation of samples to be tested
[0166] Plasma samples are used as samples to be tested without pretreatment;
[0167] 3) Blank sample
[0168] Described water is used as blank sample, and its ammonia (ammonia ion) concentration is 0 micromole / liter;
[0169] B. Preparation of reagent solution:
[0170] Ammonia (ammonia ion) diagnostic / determination reagent is a two-dose reagent, which contains:
[0171] Reagent 1 with the following composition:
[0172] Phosphate buffer 100mmol / L
[0173] NADH0.25mmol / L
[0174] Adenosine triphosphate 5mmol / L
[0175] Carbamoyl phosphate 5mmol / L
[0176] Monovalent phosphate 5mmol / L
[0177] Acetyl-CoA 5mmol / L;
[...
Embodiment 3
[0200] Embodiment 3: the mensuration of ammonia (ammonia ion) content in plasma sample
[0201] The mode of operation of this embodiment is the same as embodiment 2, but this embodiment:
[0202] B. Preparation of reagent solution:
[0203] Ammonia (ammonia ion) diagnostic / assay reagent is a three-dose reagent that contains:
[0204] Reagent 1 with the following composition:
[0205] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0206] NADH0.25mmol / L
[0207] Adenosine triphosphate 5mmol / L
[0208] Carbamoyl phosphate 5mmol / L
[0209] Monovalent phosphate 5mmol / L
[0210] Acetyl-CoA 5mmol / L;
[0211] Reagent 2 with the following composition:
[0212] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0213] Sodium chloride 2mol / L
[0214]Carbamyl phosphate synthase 28000U / L
[0215] Pyruvate dehydrogenase 50000U / L;
[0216] Reagent 3 with the following composition:
[0217] Tris(carboxymethyl)aminomethane-hydrochloric acid ...
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