Determination method of ammonia (ammonia ion) and ammonia (ammonia ion) diagnosis/determination kit
A technology of ammonia ions and reagents, which is applied in the field of medical/food testing and determination, and can solve the problems of undiscovered blood ammonia determination methods, etc.
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Embodiment 1
[0136] Embodiment 1: the mensuration of ammonia (ammonia ion) content in blood plasma
[0137] 1) Preparation of standard samples
[0138] Dissolve a certain amount of ammonia salt in water or buffer solution, and then adjust the ammonia concentration to 100 micromole / liter;
[0139] 2) Pretreatment of samples to be tested
[0140] Plasma samples are used as samples to be tested without pretreatment;
[0141] 3) Blank sample
[0142] Described water or buffer solution are used as blank sample, and its ammonia concentration is 0 micromole / liter;
[0143] B. Preparation of reagent solution:
[0144] The ammonia diagnosis / measurement reagent of the present embodiment is a single-dose reagent, which contains:
[0145] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0146] Glycerin 1mol / L
[0147] NADP + 0.25mmol / L
[0148] Ammonia kinase 16000U / L
[0149] Carbamyl phosphate synthase 18000U / L
[0150] Fatty acyl-CoA synthe...
Embodiment 2
[0169] Embodiment 2: the mensuration of ammonia (ammonia ion) content in blood plasma
[0170] 1) Preparation of standard samples
[0171] A certain amount of ammonia salt is dissolved in water, and then the concentration of ammonia (ammonia ion) is adjusted to 100 micromole / liter, as a standard sample;
[0172] 2) Preparation of samples to be tested
[0173] Plasma samples are used as samples to be tested without pretreatment;
[0174] 3) Blank sample
[0175] Described water is used as blank sample, and its ammonia (ammonia ion) concentration is 0 micromole / liter;
[0176] B. Preparation of reagent solution:
[0177] Ammonia (ammonia ion) diagnostic / determination reagent is a two-dose reagent, which contains:
[0178] Reagent 1 with the following composition:
[0179] Phosphate buffer 100mmol / L
[0180] NAD + 0.25mmol / L
[0181] Adenosine triphosphate 5mmol / L
[0182] Glutamic acid 5mmol / L
[0183] Carbamoyl phosphate 5mmol / L
[0184] Coenzyme A 5mmol...
Embodiment 3
[0209] Embodiment 3: the mensuration of ammonia (ammonia ion) content in plasma sample
[0210]The mode of operation of this embodiment is the same as embodiment 2, but this embodiment:
[0211] B. Preparation of reagent solution:
[0212] Ammonia (ammonia ion) diagnostic / assay reagent is a three-dose reagent that contains:
[0213] Reagent 1 with the following composition:
[0214] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0215] NAD + 0.25mmol / L
[0216] Adenosine triphosphate 5mmol / L
[0217] Glutamic acid 5mmol / L
[0218] Carbamoyl phosphate 5mmol / L
[0219] Coenzyme A 5mmol / L
[0220] Long-chain-acyl-CoA 5mmol / L
[0221] Monovalent phosphate 5mmol / L;
[0222] Reagent 2 with the following composition:
[0223] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0224] Sodium chloride 2mol / L
[0225] Carbamyl phosphate synthase 28000U / L
[0226] Fatty acyl-CoA synthetase 50000U / L;
[0227]...
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