Novel animal source-free and feed layer-free human pluripotent stem cell culture system

A technology for human pluripotent stem cells and pluripotent stem cells, applied in the field of cell culture, can solve problems such as clinical application obstacles, and achieve the effects of low cost, wide source and low preparation cost
CN102586176BInactive Publication Date: 2013-05-08INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
Publication Date
2013-05-08
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The invention relates to a novel animal source-free and feed layer-free human pluripotent stem cell culture system. Extracellular matrix protein, namely a humanized matrix, which can support long-term culture of human embryonic stem cells and is extracted from placenta by a pepsase digestion and urea extraction method, a component prepared from human plasma by a NaCl precipitation method is prepared into a humanized medium, and the humanized medium and the humanized matrix form the animal source-free and feed layer-free culture system together. The culture system can maintain the potentialityof self-renewal and differentiation of human embryonic stem cells for a long term, and an animal source-free human induced pluripotent stem cell clone can be established in the culture system, so theanimal source-free and feed layer-free culture system which is low in cost and suitable for amplification culture lays a foundation for clinical application of pluripotent stem cells.
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Description

technical field

[0001] The invention relates to the technical field of cell culture, in particular to a novel human pluripotent stem cell culture system without animal source and feeder layer, which is suitable for long-term culture of human pluripotent stem cells. Background technique

[0002] The typical characteristic of human pluripotent stem cells is that they continuously self-renew in vitro while maintaining the potential to differentiate into cells of various germ layers. Because the establishment of human embryonic stem cells involves ethical and legal issues, its research and application are subject to certain restrictions. Induced pluripotent stem cells have solved the ethical controversy of human embryonic stem cells well. At the same time, because they have similar characteristics to human embryonic stem cells, that is, self-renewal and differentiation potential, they have brought great potential to regenerative medicine, especially personalized treatment. hope...

Claims

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