An automatic focusing system and method for a sequencing and mapping equipment
An automatic focusing and sequencing technology, applied in the field of optical imaging, can solve problems such as inaccurate automatic focusing and automatic focusing failure
Active Publication Date: 2015-09-02
WUHAN CONSIDERIN GENE & HEALTH TECH CO LTD
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[0007] The purpose of the present invention is to provide an automatic focusing system and method for sequencing and mapping equipment, aiming to solve the problem of automatic focusing failure caused by inaccurate automatic focusing in the prior art
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[0059] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.
[0060] figure 1 A schematic structural diagram of the autofocus system of the sequencing and mapping equipment in an embodiment of the present invention is shown, and the system includes: an excitation light source 1 , a filter device 2 , a distance adjustment device 3 , an imaging device 4 and an image acquisition device 5 . in:
[0061] (1) The excitation light source 1 is located on one side of the filter device 2 and is used to provide excitation light.
[0062] The excitation light source is excitation light capable of exciting fluorescent substances to emit light. There are one or more excitation light sources. Preferably, a mercury lamp light source or a laser.
[0063] (2) The filter device 2 is located on one side of the imaging device...
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The invention relates to the field of optical imaging, and particularly provides an automatic focusing system of sequencing and image collecting equipment and a method thereof. The system includes an image collecting device, an imaging device, an optical filtering device, an excitation light source, a distance adjusting device and an analyzing device. The method includes the following steps: A, the excitation light emitted by the excitation light source reaches a sequencing reaction cavity through the optical filtering device to be imaged by the imaging device, and the image is collected by the image collecting device to form an image; B, the distance adjusting device adjusts the distance, and the step A is repeated, so as to obtain multiple images; C, the analyzing device filters the interference signals of the multiple images, and the automatic focusing analysis is carried out on the collected multiple images through the signal distribution curve, so as to obtain the optimum focusing distance; and D, the analyzing device controls the distance adjusting device to adjust the distance between the sequencing reaction cavity and the imaging device, so as to obtain the optimum distance. By adopting the system and the method, the focusing of the sequencing and image collecting equipment is more precise.
Description
technical field [0001] The present invention relates to the field of optical imaging, and more specifically, relates to an automatic focusing system and a method thereof for sequencing and image acquisition equipment. Background technique [0002] The sequencing image acquisition equipment is used to collect images in the sequencing reaction chamber to facilitate subsequent data analysis and processing. [0003] In one prior art, an automatic focusing system of a sequencing and mapping equipment includes: a microscope, a camera and a light source. The light emitted by the light source is directly irradiated into the sequencing reaction chamber through the microscope, and the camera collects images of the sequencing reaction chamber through the microscope, wherein the sequencing reaction chamber is used to fix the sample to be tested and provide a place for the sequencing reaction to occur. In this technical solution, the light emitted by the light source directly shines int...
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IPC IPC(8): G03B13/36G02B7/36G01N21/64
Inventor 盛司潼
Owner WUHAN CONSIDERIN GENE & HEALTH TECH CO LTD