Primer for detecting pytophthora capsici developed on basis of plygalacturonase Pcipg8 gene and method therefor

Abstract

The invention discloses a primer for detecting pytophthora capsici developed on the basis of plygalacturonase Pcipg8 gene and a method for detecting the pytophthora capsici. The primer for detecting the pytophthora capsici developed on the basis of the plygalacturonase Pcipg8 gene consists of single-strained DNA (deoxyribonucleic acid) shown by a sequence 1 in a sequence table and single-strained DNA shown by a sequence 2 in the sequence table. The primer can be used for carrying out qualitative and quantitative molecule detection on the phytophthora capsici leonian and different phytophthoracapsici leonian materials at different periods, and detecting and easily warning the primary infection source of the phytophthora capsici leonian and the dynamic status of the growth and decline of the germs at different periods in the process of infection, so that the optimum disease preventing and controlling period can be conveniently and timely confirmed, particularly the comprehensive prevention, control and treatment can be timely carried out in the early activity period of the germs, according to the existence and the quantity for detecting the oospore in the soil, the soil can be timely treated, the breeding and spreading of source germs can be cut off, and the disease preventing and controlling cost can be reduced.

Description

technical field [0001] The invention relates to a primer and a method for detecting Phytophthora capsici developed based on polygalacturonase Pcipg8 gene. Background technique [0002] Phytopathogenic oomycetes are an important class of plant pathogens, which can infect and harm a variety of plants and cause a variety of plant-destructive diseases, such as Phytophthora infestans, P. capsici, soybean blight P. sojae, P. parasitic, P. cinnamomi and P. hibemalis cause important diseases of potato, pepper, soybean, tobacco, camphor tree and citrus respectively. Serious years and even extinction. Oomycetes mainly use mycelium or oospores to survive the adverse environment in the diseased residue or soil and serve as the source of primary infection. Under suitable conditions, mycelium or oospores can germinate to produce sporangia-release zoospores , spread and infect by rain or air currents and cause plant diseases. Therefore, early and timely inspection and control of disease...

AI Technical Summary

Problems solved by technology

Traditional disease prevention and control strategies mainly rely on control measures such as varieties, cultivation, chemical control, and ecological regulation. Comprehensive prevention and control and high-efficiency management measures are short and get twice the result with half the effort. The control effect is very small, and it is difficult to control the occurrence and prevalence of diseases in the end.

[0003] In recent years, PCR technology and its related molecular detection technology have been widely used in the molecular detection and early warning of the growth and decline dynamics of plant pathogens. Ribosomal rDNA / ITS sequences are used to design specific primers, and targeted researches on Phytophthora soybean and Phytophthora infestans have been carried out. Molecular detection technology research on mildew, Phytophthora winteris, Phytophthora parasitica, and Phytophthora capsici, but these technical R&D achievements can only detect and warn the activity dynamics of the pathogens, but cannot detect and warn the pathogenic characteristics and occurrence trends of the pathogens

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