Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for separating, purifying and culturing human blood and marrow dendritic cells

A technology of dendritic cells and bone marrow, which is applied in the field of biomedicine, can solve problems such as the inability to efficiently expand limited dendritic cells, and achieve the effect of simple and clear operation process and shortened operation time

Active Publication Date: 2014-12-24
天津市灏洋生物制品科技有限责任公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention proposes a method for separating, purifying and culturing dendritic cells from human blood and bone marrow, which solves the problem that limited dendritic cells cannot be efficiently expanded in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating, purifying and culturing human blood and marrow dendritic cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0037] Such as figure 1 As shown, a method for separating, purifying and culturing human blood and bone marrow dendritic cells comprises the following steps:

[0038] 1. Use the diatrizoate solution with a mass fraction of 34% and the hydroxyethyl starch 550 solution with a mass fraction of 9% to prepare a mixture with a specific gravity of 1.065-1.077g / ml to separate human blood and bone marrow dendritic cells liquid, this step is specifically:

[0039] 1.1. Wei...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for separating, purifying and culturing human blood and marrow dendritic cells, which includes the following steps that: hydroxyethyl starch and meglumine diatrizoate are used for preparing human blood and marrow dendritic cell-separating solution; sodium carbonate, sodium bicarbonate and CD123 antibody are used for producing a human blood and marrow dendritic cell culture flask; L-proline, insulin, vitamin C, L-glutamine, human umbilical cord blood plasm, recombinant human fibroblast growth factor and DMEM / F12 basic medium are used for preparing human blood and marrow dendritic cell culture solution; and human blood and marrow dendritic cells are separated, purified and cultured. The method for separating, purifying and culturing human blood and marrow dendritic cells can effectively prepare a large quantity of human blood and marrow dendritic cells, and is divided into four parts, and compared with the conventional method, the method has the advantages of simple and clear operation process and shorter operation time.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for separating, purifying and culturing human blood and bone marrow dendritic cells. Background technique [0002] Dendritic cells (dendritic cells; DC) are one of the antigen-presenting cells (APCs) present in peripheral blood, skin, lymphoid organs and thymus, and are enriched in lymphoid and non-lymphoid tissues. Dendritic cells have a strong ability to present antigens, which can express antigenic peptides to class I and class II dendritic cells, and activate CD4 and CD8 T cells, respectively, thereby inducing in vivo response to specific antigens (pathogenic microbial antigens) , tumor antigen, transplant antigen, etc.) immune response. The powerful immune-inducing function of DC is very suitable for immunotherapy (DC treatment) of various tumors. Previous reports have shown that DCs stimulated with Sendai virus (SeV) have strong anti-tumor effects in mouse ex...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0784
Inventor 高旭
Owner 天津市灏洋生物制品科技有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products