Method for separating, purifying and culturing human blood and marrow dendritic cells
A technology of dendritic cells and bone marrow, which is applied in the field of biomedicine, can solve problems such as the inability to efficiently expand limited dendritic cells, and achieve the effect of simple and clear operation process and shortened operation time
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[0036] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.
[0037] Such as figure 1 As shown, a method for separating, purifying and culturing human blood and bone marrow dendritic cells comprises the following steps:
[0038] 1. Use the diatrizoate solution with a mass fraction of 34% and the hydroxyethyl starch 550 solution with a mass fraction of 9% to prepare a mixture with a specific gravity of 1.065-1.077g / ml to separate human blood and bone marrow dendritic cells liquid, this step is specifically:
[0039] 1.1. Wei...
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