Method for technically propagating dual-purpose genic male sterile rice at high altitudes

A nuclear sterile line and dual-purpose technology, applied in rice cultivation, botany equipment and methods, applications, etc., can solve problems such as narrow fertile temperature range, failure of breeding seeds, safety production restricting the development of two-line hybrid rice, etc.

Inactive Publication Date: 2012-08-29
YUNNAN JINRUI SEED IND
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

However, restricted by the physiological critical low temperature, the fertile temperature range of the dual-purpose GMS line with a low sterility threshold temperature in the fertile sensitive period is very narrow (19°C to 21°C), which makes it possible to breed in situ under natural conditions. extremely difficult
However, the utilization of spring propagation in Hainan and cold water series irrigation can solve the problem of temporary multiplication of dual-purpose GMS lines to a certain extent, but increases the cost of multiplication and will lead to multiplication failure if a little carelessness ( The success rate of breeding in Hainan is less than 40%)
Therefore, the breeding of dual-purpose GMS lines and the safe production of two-line hybrid rice seeds have become the main bottleneck restricting the development of two-line hybrid rice.

Method used

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  • Method for technically propagating dual-purpose genic male sterile rice at high altitudes

Examples

Experimental program
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Effect test

Embodiment 1

[0010] Example 1: Taking the japonica rice dual-purpose GMS lines Yunjing 202, Yun 20115S, Yun 20672S and N95076S selected or introduced by the applicant as examples, the implementation of plateau seed propagation technology is described.

[0011] 1. CMS characteristics

[0012] For the japonica rice dual-purpose male sterile line selected in Example 1, the sterility threshold temperature is all in the range of 21°C to 23°C. Its fertility sensitive period is required to be arranged around August 10, so that its fertility can basically return to normal.

[0013] 2. Plateau breeding technology

[0014] Through the research on the law of fertility conversion of photothermosensitive male sterile lines in different ecological regions of Yunnan. Select 4 pilot sites in Shuifu, Yuxi, Mile and Baoshan in Yunnan, 5 altitude conditions (300m ~ 1650m), and conduct staged sowing experiments under the natural conditions of the field. The sterile line was transplanted when the leaf age w...

Embodiment 2

[0019] Embodiment 2: Taking the introduction of the indica rice dual-purpose GMS line C815S selected and bred by Hunan Agricultural University as an example,

[0020] Describe the implementation of plateau breeding technology.

[0021] 1. CMS characteristics

[0022] C815S is a rice dual-purpose male sterile line bred by the Rice Research Institute of Hunan Agricultural University, and its sterility threshold temperature is between 21°C and 23°C. Its fertility sensitive period is required to be arranged around August 10, so that its fertility can basically return to normal.

[0023] 2. Plateau breeding technology

[0024] In view of the results of the japonica-type dual-purpose GMS line breeding test, in 2010, the indica-type dual-purpose GMS line C815 was used as a test material, and three altitude points (1340m, 1540m and 1650m) were selected for conventional cultivation and management. The method was further verified in the low latitude and high altitude area of ​​Baosha...

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Abstract

The invention relates to a high-production low-danger propagation technology of a photo thermo sensitive genic male sterile rice, in which the propagation cost and the danger of the photo thermo sensitive genic male sterile rice are lowered by utilization of the natural low-temperature condition at high altitudes, the difficulties in propagating the dual-purpose genic male sterile rice are avoided and the development of a two-line hybrid rice is promoted. The method comprises the following steps: selecting a propagation base at appropriate altitudes that the latitude is 24 degree to 26 degree and the altitude is 1500 m to 1650 m; arranging a sowing season that the heading and flowering stage can be on about August 10th and the seeding time is generally on April 1st to April 16th at the altitudes of 1500 m to 1650 m; implementing the cultivation management technology that transplant is carried out when the sterile line leaf age is 4-5 leaves, compact planting is conducted moderately, the planting space is generally 15cm*20cm or 13cm*15cm for single-plant interposition; and arranging temperature reduction facilities in abnormal temperature in areas with favorable irrigation conditions, so that the temperature can be lowered by cold water in a mountain spring once the external temperature becomes abnormal and can not meet the temperature requirement of the sterile line in the sensitive stage.

Description

Technical field: [0001] The invention relates to a high-yield and low-risk high-yield propagation technology of a rice photothermosensitive genetic male sterile line. The proposal of this scheme is to take advantage of the natural low temperature conditions in high altitude areas, reduce the cost and risk of the reproduction of rice photothermo-sensitive genetic male sterile lines, and will promote the development of two-line hybrid rice to a certain extent. Background technique: [0002] The two-line hybrid rice has been highly valued by experts and scholars in the field of rice breeding because of its advantages such as free mating, easy breeding, extremely wide recovery spectrum and no negative cytoplasmic effects of sterility. Compared with the three-line method, hybrid rice with two-line method has great potential for high yield. Judging from the dual-purpose genetic male sterile line selected so far, we have bred a photothermo-sensitive genetic male sterile line that c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G16/00A01G22/22
Inventor 卢义宣涂建刘晓利奎丽梅辜琼瑶李华慧杨久黄平张云
Owner YUNNAN JINRUI SEED IND
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