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Protein markers and detection methods for liver fibrosis in chronic hepatitis B

A hepatitis liver fiber and protein labeling technology, applied in the field of biomedicine, to achieve the effect of improving specificity and sensitivity

Inactive Publication Date: 2015-11-25
上海交通大学附属第一人民医院
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] However, there is currently no report using the serum protein of the present invention as a marker for the degree of liver fibrosis in chronic hepatitis B

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  • Protein markers and detection methods for liver fibrosis in chronic hepatitis B

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Embodiment 1

[0027] Example 1: Verification of serum protein molecular markers in diagnosing the degree of liver fibrosis in chronic hepatitis B.

[0028] The serum protein molecular markers of the present invention collected 45 cases of liver fibrosis from chronic hepatitis B at different stages of liver fibrosis (Scheuer stage S0-S4, with an average of 10 cases in each group), all of which were confirmed by liver biopsy pathological diagnosis of fibrosis According to the degree of fibrosis, the S0 and S1 stages were classified as the early fibrosis group, and the S3 and S4 stages were classified as the advanced fibrosis group, and serum samples were collected. All serum samples were detected by proteomics detection technology (2DE-MS / MALDI-ToFMS), and the electrophoresis patterns were analyzed by ImageMaster software. The results show that the expression of the serum protein markers of the present invention differs by more than 2 times between the early fibrosis group and the late fibros...

Embodiment 2

[0032] Example 2: Application of Serum Protein Molecular Markers in Diagnosis of Liver Fibrosis in Chronic Hepatitis B

[0033] 1. Research objects: 100 cases of different stages of liver fibrosis (Scheuer stages S0-S4, with an average of 20 cases in each group) were collected in 2010.

[0034] 2. Research method: Collect 100 cases of chronic hepatitis B liver fibrosis cases with different stages of liver fibrosis (Scheuer stage S0-S4, with an average of 20 cases in each group). The S0-S2 stage was classified as the early fibrosis group, and the S3-S4 stage was classified as the advanced fibrosis group, and serum samples were collected. All serum samples were detected by serum ELISA to obtain the detection value of serum protein in the early fibrosis group and the advanced fibrosis group, and the statistical analysis between the groups showed statistical differences ( p <0.05), it is positive if it is consistent with the above diagnostic markers, otherwise it is negative.

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Abstract

The invention belongs to the field of biomedicine, and relates to a non-traumatic serum protein molecular marker for diagnosing chronic hepatitis liver fibrosis. The protein marker provided by the invention is one or more of the following proteins: human serum glycosylation apoferritin-transferrin A chain, glycoprotein HC composite structure C chain, human complement component C3c, immune globulin MFc segment, human hemoglobin beta chain variant HbS wake, or protein A mimetic peptide tree-like ligand of IgG1Fc segment A chain (Herceptin). The invention also provides the detection method and application of the serum protein marker and a kit prepared according to the serum protein marker. In the invention, the serum protein molecular marker and related indexes such as mRNA and MicroRNA can be used for predicting the degree of chronic hepatitis liver fibrosis and can be used for prognosis judgment, treatment selection and curative effect evaluation.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a serum molecular marker for diagnosing hepatic fibrosis in biomedicine, in particular to a class of serum protein molecular markers for non-invasive diagnosis of chronic hepatitis B liver fibrosis. Background technique [0002] For a long time, liver biopsy has been the gold standard for determining the etiology of liver diseases and evaluating the degree of liver inflammation, necrosis, and fibrosis. It has important clinical significance for prognosis, treatment selection, and efficacy evaluation. Insufficiencies such as inter-observer bias have caused a lot of inconvenience to clinical application, and there is an urgent need to find convenient non-invasive alternative indicators in clinical diagnosis and treatment. In recent years, establishing a diagnostic model of liver fibrosis with a combination of serum indicators has become a worldwide research hotspot. Among a series of non-i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
Inventor 陆伦根徐铭益王燕曲颖董育玮蔡晓波
Owner 上海交通大学附属第一人民医院
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