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Fast seedling breeding method for tissue cultivation of callicarpa bodinieri and method for transplanting rooting seedlings of callicarpa bodinieri

A tissue culture and purple pearl technology, applied in the field of plant cultivation, can solve the problems of low reproductive rate, slow growth, large environmental impact and the like of purple pearl

Inactive Publication Date: 2012-09-19
江苏九久环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the defects in the prior art such as low reproductive rate, slow growth, poor genetic stability, and the cultivation process is greatly affected by the environment and difficult to control, and to provide a rapid seedling cultivation method of purple pearl tissue culture and its production method. The root seedling transplanting method has virus-free, high reproduction rate, and fast growth rate, which can effectively avoid the problem of species degeneration, and is convenient for management, which is conducive to automatic control and can provide a large number of seedlings in a short time

Method used

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  • Fast seedling breeding method for tissue cultivation of callicarpa bodinieri and method for transplanting rooting seedlings of callicarpa bodinieri
  • Fast seedling breeding method for tissue cultivation of callicarpa bodinieri and method for transplanting rooting seedlings of callicarpa bodinieri

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Experimental program
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Effect test

Embodiment 1

[0097] The tissue culture rapid seedling raising method of Zizhu and its rooted seedling transplanting method, the concrete steps are as follows:

[0098] (1) Take the strong branches with axillary buds of Zizhu in the same year and remove the leaves as explants. First, soak in the aqueous solution of washing powder with a concentration of 1wt% for 15 minutes, then gently scrub with a soft brush, and then put them under running water. Rinse for 3 hours; then soak in 70wt% alcohol for 45s on the ultra-clean workbench, rinse with sterile water for 4 times; then disinfect with 0.1wt% mercuric chloride for 12 minutes, rinse with sterile water for 6 times; dry the surface Cut the stem segment into 1 cm long stem segment with 1 axillary bud after watering.

[0099] (2) Inoculate the stem segment treated in step (1) into the induction medium, which is added with 0.2mg / L of 6-BA and 0.01mg / L of IBA in the MS conventional medium at the same time; Under the conditions of temperature 25...

Embodiment 2

[0104] The tissue culture rapid seedling raising method of Zizhu and its rooted seedling transplanting method, the concrete steps are as follows:

[0105] (1) Take the strong branches with axillary buds of Zizhu in the same year and remove the leaves as explants. First, soak them in an aqueous solution of washing powder with a concentration of 1wt% for 10 minutes, then gently scrub them with a soft brush, and then place them under running water. Rinse for 2 hours; then soak in 70wt% alcohol for 30s on the ultra-clean workbench, rinse twice with sterile water; then disinfect with 0.1wt% mercuric chloride for 10 minutes, rinse with sterile water 4 times; dry the surface After moisture, the stem segment is cut into a 0.8 cm long stem segment with 1 axillary bud.

[0106](2) Inoculate the stem segment treated in step (1) into the induction medium, which is added with 0.3mg / L of 6-BA and 0.02mg / L of IBA in MS conventional medium at the same time; The temperature is 22°C, the light...

Embodiment 3

[0111] Zizhu tissue culture rapid seedling raising method and rooted seedling transplanting method thereof, the concrete steps are as follows:

[0112] (1) Take the strong branches with axillary buds of Zizhu in the same year and remove the leaves as explants. First, soak them in an aqueous solution of washing powder with a concentration of 1wt% for 20 minutes, then gently scrub them with a soft brush, and then place them under running water. Rinse for 4 hours; then soak in 70wt% alcohol for 60s on the ultra-clean workbench, rinse with sterile water for 6 times; then disinfect with 0.1wt% mercuric chloride for 15 minutes, rinse with sterile water for 8 times; dry the surface Cut the stem segment into 1.2cm long stem segment with 1 axillary bud after moisture.

[0113] (2) Inoculate the stem segment treated in step (1) into the induction medium, which is added with 0.5mg / L of 6-BA and 0.04mg / L of IBA in MS conventional medium at the same time; The temperature is 28°C, the ligh...

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Abstract

The invention belongs to the plant cultivating field, relates to tissue culture of plants and particularly relates to a fast seedling breeding method for tissue cultivation of callicarpa bodinieri and a method for transplanting rooting seedlings of the callicarpa bodinieri. The method comprises the five steps of explant disinfection, inductive culture, enrichment culture, rooting culture and tissue culture plantlet transplant. The method has advantages of virus-free, high reproduction rate and fast reproduction rate, solves the problems of low reproduction rate, slow growth and poor genetic stability of the callicarpa bodinieri in the prior art, and can be used for providing a large number of seedlings in a short time. The method provided by the invention can be used for providing the finished seedlings of the callicarpa bodinieri in a large industrialized scale, thus meeting the urgent needs of landscaping and development of traditional Chinese medicine resources.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and relates to plant tissue culture, in particular to a method for rapidly growing seedlings of purple pearls through tissue culture and a method for transplanting rooted seedlings. Background technique [0002] Zizhu is a deciduous shrub belonging to Verbenaceae Zizhu. It has a beautiful plant shape, soft branches, and strong resistance to cold and shade. Planted on the edge of courtyards, parks, and green spaces, it is novel and unique, and it looks delicate and lovely when planted with evergreen trees. It is an ornamental flower and tree with excellent flowers and fruits, and is an excellent tree species for landscaping. Its roots, stems, leaves, flowers, and seeds can be used as medicine. It has unique functions such as clearing heat and detoxifying, cooling blood to stop bleeding, reducing inflammation and promoting muscle growth, and anti-cancer. The treatment of uterine bleeding, skin pur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 邓艳
Owner 江苏九久环境科技有限公司
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