Preparation process of mouse carotid artery injury model and application thereof

A damage model, carotid artery technology, applied in the direction of peroxide active ingredients, etc., can solve the problem of long distance in the pathological process of coronary thrombosis, and achieve the effect of improving scientificity and credibility, low price and good repeatability

Inactive Publication Date: 2012-09-19
JILIN UNIV
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AI Technical Summary

Problems solved by technology

Injection of thrombin, mechanical injury and physical injury can all induce arterial thrombosis, but the pathological process of coronary thrombosis is far apart

Method used

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Experimental program
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Effect test

Embodiment Construction

[0017] The preparation method of mouse carotid artery injury model of the present invention comprises the following steps:

[0018] 1. Select male C57BL / 6 mice born at 8-12 weeks and weighing 20-25g;

[0019] 2. The mice were anesthetized by intraperitoneal injection of 300 mg / kg of chloral hydrate, and the limbs of the mice were bound in a supine position after anesthesia;

[0020] 3. Disinfect the skin of the mouse carotid artery with 75% alcohol;

[0021] 4. Make a 1cm longitudinal incision along the midline of the mouse neck, fully expose the inner carotid structure, and separate one side of the carotid artery;

[0022] 5. Pick up one side of the carotid artery with elbow tweezers, wrap the carotid artery with filter paper soaked in 10% hydrogen peroxide, and stay for 3 minutes (the purpose is to damage one side of the carotid artery of the mouse);

[0023] 6. Remove the filter paper, wash the incision three times with 0.01mol / LPBS and then suture;

[0024] 7. After the...

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Abstract

The invention provides a preparation process of a mouse carotid artery injury model and an application thereof and belongs to the technical field of medical animal models. The preparation process includes: narcotizing a C57BL / 6 male mouse by injecting chloral hydrate in dosage of 300mg / kg into the abdominal cavity of the mouse, cutting along the carotid midline to form a 2-cm longitudinal incision, and covering one side of carotid artery by filter paper soaked with hydrogen peroxide to make injury on the carotid artery so as to prepare the mouse carotid artery injury model. The mouse carotid artery injury model can meet requirements for researches on forming and developing of blood clots after carotid artery internal membranes are subjected to injury, can actually simulate the pathological process of forming of blood clots after carotid artery internal membranes are subjected to injury in practical use, and lays a stable fundament for researching cardiovascular diseases in a system manner and particularly for researching forming of carotid atherosclerosis blood clots and further developing medicines for treating cardiovascular diseases and exploring therapeutic schedule and the like. In addition, the mouse carotid artery injury model can be prepared by simple operation within a short molding cycle, is high in success rate, good in repeatability, low in cost and easy to popularize and apply.

Description

technical field [0001] The invention belongs to the technical field of medical animal models, and in particular relates to a preparation method and application of a mouse carotid artery injury model. Background technique [0002] With the continuous improvement of people's living standards, the incidence of cardiovascular and cerebrovascular diseases is increasing, and its morbidity and mortality have surpassed tumor diseases and ranked first, and the important cause of cardiovascular diseases is due to thrombosis. Formation, the thrombus model is an important tool for human to study thrombotic diseases and develop anticoagulant and thrombolytic drugs. Therefore, an ideal animal thrombus model is an essential experimental tool for revealing its pathogenesis, exploring treatment methods, and developing new drugs. [0003] At present, the commonly used methods for preparing animal arterial thrombus models are thrombin injection method or arterial intima injury method (includi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/40
Inventor 丁瑜张学锋张嘉保任文陟孙红
Owner JILIN UNIV
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