The invention discloses a method for making a guinea pig MCAO (middle cerebral artery occlusion) model, which comprises the steps of preparing an anesthetic, weighing a guinea pig, and injecting a certain amount of the anesthetic into the abdominal cavity according to the weight of the guinea pig, so that the guinea pig is anesthetized; placing the guinea pig on a temperature control blanket, fixing the guinea pig on an operating table with the body of the guinea pig straight and free of tension, scraping the neck hair of the guinea pig with a hair clipper, and locally sterilizing with entoiodine; cutting the skin along the paracervical median of the guinea pig to open the anterior cervical fascia and expose the sternocleidomastoid muscle on the right side, then separating the anterior cervical muscle group and sternocleidomastoid muscle, and drawing with a fine silk thread to separate the muscle tissues until a complete CCA (common carotid artery) is observed. Compared with the traditional common carotid artery plug-in method, the method has the advantages that blood is directly supplied from the common carotid artery through the internal carotid artery after reperfusion, the common carotid artery is directly ligated after reperfusion, the reperfusion blood supply can only be circulated through lateral branches, the reperfusion effect similar to the clinical effect can be achieved, and the success rate of model preparation is high.