Method for establishing rat external carotid artery catheter-indwelled cerebral ischemia reperfusion model

Abstract

The invention relates to a method for establishing a rat external carotid artery catheter-indwelled cerebral ischemia reperfusion model. The method includes the following steps that: the direction of an external carotid artery blood vessel of a rat is drifted, the direction of the external carotid artery blood vessel of the rat is drifted to a position which is consistent with or parallel to the direction of an internal carotid artery blood vessel of the rat; the external carotid artery blood vessel is cut, a suture is inserted into an incision, so that cerebral ischemia can be formed in the neck of the rat; and 1 to 2 hours after the suture is inserted into the incision, and cerebral ischemia is formed, the suture is pulled out, and a catheter injection bag through which medicine can be injected repeatedly is arranged into the external carotid artery blood vessel. According to the method, an external carotid artery suture method is adopted to manufacture the rat ischemia reperfusion model, and therefore, the normal structures of an operation-side common carotid artery and an internal carotid artery can be protected; the catheter injection bag through which medicine can be injected repeatedly is indwelled in the external carotid artery, medicine can be injected into the internal carotid artery through the artery catheter repeatedly at different time points; nerve functional assessment after the model is established can be realized, and qualified animals could be accepted and subjected to experimental intervention, and therefore, the scientific nature of experiments can be greatly improved.

Description

technical field [0001] The invention belongs to the technical field of medical animal experiments, and specifically relates to a method for establishing a rat cerebral ischemia-reperfusion model with a preserved external carotid artery catheter. Background technique [0002] Cerebrovascular disease is a common clinical disease with high disability rate and high mortality rate. Among them, ischemic cerebrovascular disease is the most common case, and it is also a key research and urgent problem to be solved by domestic and foreign medical experts and scientific researchers. The current method is to explore new treatment methods through animal experiments, that is to say, through animal experiments, a model similar to human diseases is established in animals, in an attempt to find a solution to deep-seated treatment of cerebrovascular diseases. . [0003] At present, the main method of most animal experiments is to block the middle artery of the animal rat brain for a short t...

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Problems solved by technology

[0005] 1. Since an incision was made on the common carotid artery of the rat, and then a thread embolism was inserted to form arterial occlusion, the common carotid artery was permanently occluded, which destroyed the normal hemodynamics in the blood vessel. Cases that conform to the clinical reality of the disease and cannot be used as clinical reference

[0006] 2. In some experimental cases, it is necessary to inject drugs or biological agents into the internal carotid artery of rats. However, due to the occlusion of the internal carotid artery formed by the "suture method", it is impossible to preserve the arterial injection route. Some researchers have to use In the early stage of model establishment, the internal carotid artery was directly injected with drugs and then occluded. However, after the internal carotid artery is occluded, the researchers could not inject drugs into the artery again or repeatedly at different time points, and they could not predict Judging whether the modeling is successful will have a great impact on the conclusion of the research, so it cannot (cannot) be a case for clinical reference

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