Implantable internal carotid artery constant-speed injection device and operation method thereof

Abstract

The invention discloses an implantable internal carotid artery constant-speed injection device and an operation method thereof. A sealing cover and a sealing plug which not only have a sealing effect,but also can be pierced by a needle are separately arranged at the top end and the rear end of a common carotid artery implanting guide tube, an injection opening which is covered by a one-way circulating door is formed in the front end of the common carotid artery implanting guide tube, flow speed and time are controlled through a controller, a constant-speed injection micro-pump is driven to push an injection syringe, and cancer cells or injection drugs flow into common carotid artery blood vessels through an injection opening of the common carotid artery implanting guide tube. By the implantable internal carotid artery constant-speed injection device, a surgical method can be simple and convenient, surgical steps are simplified, and surgery time is shortened; and meanwhile, a surgery mode of for an animal model of mouse internal carotid artery injection will be changed completely, the external carotid artery of a mouse does not need to be sutured, pneumogastric nerves and blood vessels cannot be damaged, and the success rate of the surgery is greatly increased.

Description

technical field [0001] The invention relates to mouse animal model technology, in particular to an implantable internal carotid artery constant-velocity injection device and an operating method thereof. Background technique [0002] When making mouse animal models of brain metastasis of various cancer cells, it is usually necessary to ligate the proximal end of the common carotid artery and the external carotid artery of the mouse, and then use a syringe to inject cancer cells cultured in vitro from the distal end of the common carotid artery. The needle is injected into the internal carotid artery to reach the blood-brain barrier, and then spontaneously transferred to the brain to create a mouse animal model most similar to human cancer brain metastasis. According to the local anatomy, the common carotid artery is divided forward into the external carotid artery and the internal carotid artery, and only the blood in the internal carotid artery flows into the cranium. There...

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Problems solved by technology

In addition, after the successful ligation of the external carotid artery, the needle of the syringe filled with cancer cells needs to be inserted into the distal end of the common carotid artery (above the ligation line at the proximal end), and then the cultured cancer cells in vitro should be injected into the internal carotid artery. The process needs to strictly control the injection speed, that is, when the concentration of cancer cells is constant, the faster the injection speed, the more cancer cells will reach the blood-brain barrier per unit time, which will artificially cause tumor embolus and block blood vessels, which is equivalent to causing small The mice died of extensive thrombotic disease, which is another important reason for the death of mice with failed surgery

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