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Method for efficiently preparing inhomogenous Marasmius androsaceus polysaccharide through membrane separation technology

A technology of Anluo Xiaopi umbrella and polysaccharides, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemicals for biological control, etc., can solve the problems of high labor intensity, high production cost, and destruction of active ingredients, etc. problems, to achieve the effect of reducing labor costs, high degree of automation, and long shelf life

Inactive Publication Date: 2014-04-09
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the complex production process and high cost of the existing one-class heterogeneous Anluo Xiaopi umbrella polysaccharide production method, low polysaccharide content, many impurities in the fermented liquid, and the effective use of chemicals on the product. ingredients causing damage etc.
It is used to solve the problems of high labor intensity, low production efficiency, serious environmental pollution, high production cost, poor product quality and low product yield in the prior art

Method used

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  • Method for efficiently preparing inhomogenous Marasmius androsaceus polysaccharide through membrane separation technology
  • Method for efficiently preparing inhomogenous Marasmius androsaceus polysaccharide through membrane separation technology
  • Method for efficiently preparing inhomogenous Marasmius androsaceus polysaccharide through membrane separation technology

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specific Embodiment approach 1

[0010] In this embodiment, a membrane separation technology is used to separate, extract and concentrate a class of heterogeneous polysaccharides from the fungus Coprinus parvum from the fermentation broth according to the following steps:

[0011] 1. Obtain the fermented liquid after submerging the Anluo Xiaopi umbrella producing Anluo Xiaopi polysaccharide;

[0012] 2. Freeze-dry the fermented liquid in step 1 to a constant mass, crush it with a pulverizer, and pass through a 60-mesh sieve;

[0013] 3. Extract the quasi-dry powder in step 2 with distilled water in a constant temperature water bath at 70°C for 2 hours;

[0014] 4. Concentrate and purify the extract in the extract through an inorganic ceramic microfiltration membrane with a molecular weight cut-off of 0.01-0.03um at an operating temperature of 25-45°C and a membrane flux of 50-60LMH Heterogeneous Anluo parsley polysaccharide, the solution passed is Anluo parsley polysaccharide solution, the concentration fact...

specific Embodiment approach 2

[0028] The difference between this embodiment and the specific embodiment 1 is that in step 1, a type of heterogeneous umbrellas are inoculated into a fermenter for fermentation and cultivation, the fermentation temperature is 25-28°C, and the fermentation time is 60-70h. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0029] The difference between this embodiment and the specific embodiment 1 or 2 is that the concentration ratio of the polysaccharide solution of the umbrella class polysaccharide in step 4 is 7 times. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention discloses a method for efficiently preparing inhomogenous Marasmius androsaceus polysaccharide through a membrane separation technology. The method comprises the following steps: 1, performing submerged fermentation to obtain a fermentation liquor; 2, freeze-drying the fermentation liquor to reach constant mass, crushing by using a crusher, and sieving by using a 60-mesh sieve; 3, leaching by using a constant temperature water bath; 4, concentrating and purifying by using an inorganic ceramic microfiltration membrane; 5, concentrating and purifying by using an inorganic ceramic ultrafiltration membrane with the molecular weight cutoff of 4,000-9,000k; 6, removing proteins from an inhomogenous Marasmius androsaceus polysaccharide solution in the step 5 by using a sevage liquid; and 7, spray-drying the inhomogenous Marasmius androsaceus polysaccharide solution in the step 6 to obtain powder, thus obtaining the inhomogenous Marasmius androsaceus polysaccharide product, wherein the inlet temperature for the spray drying is 170-210DEG C and the outlet temperature is 80-95DEG C. The preparation method is low in cost; and the prepared product has high quality and long quality guarantee period and can be stored for more than two years without deterioration under the sealed condition.

Description

technical field [0001] The invention relates to a method for separating, extracting and concentrating a kind of non-uniform polysaccharides from the fermented liquid by membrane separation technology. Background technique [0002] China's agricultural products are reduced by billions of tons every year due to crop diseases, and the prevention and control of crop diseases is of great significance to ensure the stable development of the national economy and continuously improve people's living standards. The use of chemical pesticides is an effective means to reduce the loss of crop diseases, but due to the extensive use, abuse and non-degradability of pesticides, frequent incidents such as environmental pollution, ecological damage, food pollution, and human and animal poisoning have occurred, and serious 3R (residue, resistance, rampant regrowth) problems. Rice blast is one of the most serious rice diseases in rice fields all over the world, generally causing a 10%-20% redu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C08B37/00A01P3/00C12R1/645
Inventor 安德荣
Owner NORTHWEST A & F UNIV
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