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Application of corn defense-responsive gene

A fragment and sequence technology is applied in the application field of maize disease resistance-related gene NPR1 to achieve the effect of improving pathogen resistance

Inactive Publication Date: 2014-05-28
ANHUI AGRICULTURAL UNIVERSITY
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  • Description
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Problems solved by technology

There are five species in rice, maize NPR1 gene NCBI accession number NM_001154115, but the role of maize NPR1 gene in research and improvement of maize broad-spectrum disease resistance has not been reported

Method used

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  • Application of corn defense-responsive gene
  • Application of corn defense-responsive gene
  • Application of corn defense-responsive gene

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Experimental program
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Embodiment 1

[0025] 1. Materials

[0026] RNAiso Plus and AxyPrep DNA gel recovery kits were purchased from Treasure Bioengineering (Dalian) Co., Ltd.; Reverse Transcription System reverse transcription kits were purchased from Promega Company; pUC19 vector (see figure 1 ), pUCCRNAi vector (see map figure 2 ), CPB carrier (see map Figure 4 ) was purchased from Yubao Biological Engineering (Dalian) Co., Ltd.; pEASY-T3 (see map image 3 ) was purchased from Beijing Quanshijin Biotechnology Co., Ltd.; DH5α competent cells were purchased from Takara Bioengineering (Dalian) Co., Ltd., and AG10 competent Agrobacterium was purchased from Takara Company; the plant genome DNA extraction kit was Kangwei Century New Plant Genome DNA extraction kit; the herbicide basta was purchased from Shanghai Sangong.

[0027] YEP liquid medium formula: 10 g of beef extract, 10 g of yeast extract, 5 g of NaCl, pH 7.0, the corresponding fixed medium was added with 1.5% agar; 1 / 4MS medium was purchased from Sha...

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Abstract

The invention provides a separated polynucleotide, wherein the polynucleotide is selected from one of followings: a), a nucleotide sequence composed of part or all of sequences shown in SEQ ID NO.7; or b), a nucleotide sequence complemented with the nucleotide sequence limited in the above a). The invention also provides an application of the polynucleotide in increasing or decreasing salicylic acid content in a plant, reducing or improving plant sensibility to a pathogen, and provides an effective RNAi carrier, wherein the corn gene NPR1 is verified to have the effect on strengthening corn disease resistance; a novel method for improving corn resistance ability against pathogen is provided; the model plant with the corn gene NPR1 in silence can be obtained; and the application of the corn defense-responsive gene is of positive significance for researching broad spectrum disease resistance of the corn.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to the application of the corn disease resistance-related gene NPR1. Background technique [0002] Corn (Zea mays L. ) is one of the most widely distributed food crops in the world, and it is also an important economic crop. In my country, its distribution range and planting area are very large, and it is one of the main grains of the people in the dry valley areas of our country. However, the infestation of pests and diseases brings huge economic losses to human beings every year. Although traditional methods such as field management and chemical control can suppress the threat of pests and diseases to a certain extent, they are time-consuming, labor-intensive and pollute the environment, which has great limitations. With the strengthening of human awareness of environmental protection and the development of genetic engineering technology, how to use biological techno...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82C12N15/84C12N5/10A01H5/00
Inventor 程备久洪玲江海洋朱苏文赵阳马庆彭晓剑
Owner ANHUI AGRICULTURAL UNIVERSITY
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