Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Trichomonas vaginalis and candida albicans two-channel fluorescence PCR detection method and kit thereof

A technology for Trichomonas vaginalis and Candida albicans, applied in the biological field, can solve the problems of low positive rate and insufficient sensitivity of smear staining, achieve high sensitivity and specificity, prevent pollution, and improve detection efficiency

Active Publication Date: 2014-08-06
北京新沿线医药科技发展有限公司
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have shortcomings in one or more aspects of accuracy, reliability, ease of use, etc.
For example, the positive rate of smear staining method is low, and the culture method takes 3-7 days and is not sensitive enough

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Trichomonas vaginalis and candida albicans two-channel fluorescence PCR detection method and kit thereof
  • Trichomonas vaginalis and candida albicans two-channel fluorescence PCR detection method and kit thereof
  • Trichomonas vaginalis and candida albicans two-channel fluorescence PCR detection method and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Real-time fluorescent PCR primers and Taqman probes and screening for dual-channel detection of Trichomonas vaginalis RNA and Candida albicans DNA.

[0021] Data from GenBank

[0022] (http: / / www.ncbi.nlm.nih.gov / genomes / FLU / FLU.html) Download the complete rRNA gene sequence of Trichomonas vaginalis subtypes, and compare and analyze the consistency of all Trichomonas vaginalis rRNA gene sequences , choose a relatively conserved region to design primers and probes; the selection principle of the conserved region is: in the sequence homology analysis, the region with high conservation and suitable for PCR-specific amplification is used as the target amplification region.

[0023] Data from GenBank

[0024] (http: / / www.ncbi.nlm.nih.gov / genomes / FLU / FLU.html) Download the entire L1A1 sequence of Candida albicans for comparison and select the conserved region as the target amplification region.

[0025] Both Trichomonas vaginalis and Candida albicans target ampli...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to primers and TaqMan probes used for carrying out two-channel fluorescence PCR detection of trichomonas vaginalis viruses and candida albicans viruses. The primers and the TaqMan probes are trichomonas vaginalis virus primers and trichomonas vaginalis virus TaqMan probes designed based on a trichomonas vaginalis virus conserved area and candida albicans virus primers and candida albicans virus TaqMan probes designed based on a candida albicans virus conserved area. Simultaneously, the primers and the TaqMan probes are used for detecting whether trichomonas vaginalis virus RNA and candida albicans virus DNA exist in a sample to be detected. The invention further comprises a detection method employing the primers and the TaqMan probes and a kit containing the primers and the TaqMan probes.

Description

technical field [0001] The invention relates to a molecular biology detection method in the field of biotechnology, in particular to double detection of Trichomonas vaginalis and Candida albicans. Background technique [0002] Trichomonas is a protozoa with flagellates. Three species of Trichomonas have been found to parasitize human body, namely Oral Trichomonas, Trichomonas hominis and Trichomonas vaginalis (Trichomonas vaginalis, TV). The first two are meaningless in human etiology, while TV parasitizes in the vagina, urethra or bladder, and can cause human genitourinary tract diseases, called trichomoniasis. Trichomoniasis is divided into trichomonas urethritis, trichomonas vaginitis, trichomonas prostatitis. Female vaginitis caused by Trichomonas vaginalis is typically manifested by foamy vaginal discharge. It has been reported that Trichomonas vaginalis can swallow sperm, cause infertility, and can also cause abnormal cell development and abnormal nucleus. Therefore,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11C12R1/725
Inventor 刘巨波熊丽娟张颖吴咏今张陆明
Owner 北京新沿线医药科技发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com