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Trichomonas vaginalis and candida albicans two-channel fluorescence PCR detection method and kit thereof

A technology for Trichomonas vaginalis and Candida albicans, applied in the field of molecular biology detection, double detection of Candida albicans, and Trichomonas vaginalis, can solve the problems of low positive rate and insufficient sensitivity of smear staining method, and achieve high sensitivity and effects of high specificity, contamination prevention, high accuracy and precision

Active Publication Date: 2012-10-10
北京新沿线医药科技发展有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

These methods have shortcomings in one or more aspects of accuracy, reliability, ease of use, etc.
For example, the positive rate of smear staining method is low, and the culture method takes 3-7 days and is not sensitive enough

Method used

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  • Trichomonas vaginalis and candida albicans two-channel fluorescence PCR detection method and kit thereof
  • Trichomonas vaginalis and candida albicans two-channel fluorescence PCR detection method and kit thereof
  • Trichomonas vaginalis and candida albicans two-channel fluorescence PCR detection method and kit thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Example 1. Real-time fluorescent PCR primers and Taqman probes and screening for dual-channel detection of Trichomonas vaginalis RNA and Candida albicans DNA.

[0021] Data from GenBank

[0022] (http: / / www.ncbi.nlm.nih.gov / genomes / FLU / FLU.html) Download the complete rRNA gene sequence of Trichomonas vaginalis subtypes, and compare and analyze the consistency of all Trichomonas vaginalis rRNA gene sequences , choose a relatively conserved region to design primers and probes; the selection principle of the conserved region is: in the sequence homology analysis, the region with high conservation and suitable for PCR-specific amplification is used as the target amplification region.

[0023] Data from GenBank

[0024] (http: / / www.ncbi.nlm.nih.gov / genomes / FLU / FLU.html) Download the entire L1A1 sequence of Candida albicans for comparison and select the conserved region as the target amplification region.

[0025] Both Trichomonas vaginalis and Candida albicans target ampli...

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Abstract

The invention relates to primers and TaqMan probes used for carrying out two-channel fluorescence PCR detection of trichomonas vaginalis viruses and candida albicans viruses. The primers and the TaqMan probes are trichomonas vaginalis virus primers and trichomonas vaginalis virus TaqMan probes designed based on a trichomonas vaginalis virus conserved area and candida albicans virus primers and candida albicans virus TaqMan probes designed based on a candida albicans virus conserved area. Simultaneously, the primers and the TaqMan probes are used for detecting whether trichomonas vaginalis virus RNA and candida albicans virus DNA exist in a sample to be detected. The invention further comprises a detection method employing the primers and the TaqMan probes and a kit containing the primers and the TaqMan probes.

Description

technical field [0001] The invention relates to a molecular biology detection method in the field of biotechnology, in particular to double detection of Trichomonas vaginalis and Candida albicans. Background technique [0002] Trichomonas is a protozoa with flagellates. Three species of Trichomonas have been found to parasitize human body, namely Oral Trichomonas, Trichomonas hominis and Trichomonas vaginalis (Trichomonas vaginalis, TV). The first two are meaningless in human etiology, while TV parasitizes in the vagina, urethra or bladder, and can cause human genitourinary tract diseases, called trichomoniasis. Trichomoniasis is divided into trichomonas urethritis, trichomonas vaginitis, trichomonas prostatitis. Female vaginitis caused by Trichomonas vaginalis is typically manifested by foamy vaginal discharge. It has been reported that Trichomonas vaginalis can swallow sperm, cause infertility, and can also cause abnormal cell development and abnormal nucleus. Therefore,...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12R1/725
Inventor 刘巨波熊丽娟张陆明张颖
Owner 北京新沿线医药科技发展有限公司
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