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Rapid seedling method of medicinal sarcopyramis bodinieri calluses

A technology for clumps and spikes, which is applied to the field of rapid seedling growth of medicinal clumps of clumps, and can solve the problems of low natural reproduction coefficient and low survival rate of clumps.

Inactive Publication Date: 2012-10-17
FUJIAN AGRI & FORESTRY UNIV
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AI Technical Summary

Problems solved by technology

The natural reproduction coefficient of S. spica is low, and the survival rate of artificial cultivation by ramets or cuttings is not high

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0020] (1) Disinfection of materials: Cut off the tender stem sections with terminal buds or tender stem sections with nodes from the wild Sagittarius plant, and rinse the materials with tap water; operate under aseptic conditions, first disinfect with 75% alcohol for 10s, and then Soak in 0.1% mercuric solution for 10 min, and finally rinse with sterile water for 5 times. Drain the water and put it in a sterile petri dish for later use;

[0021] (2) Culture medium:

[0022] 1) Preparation of media for three different growth stages:

[0023] Cluster bud induction medium: MS + 0.5mg / L BA + 0.2mg / L NAA, white sugar 30 g / L, agar 0.7%, pH 5.6.

[0024] Cluster bud proliferation medium: MS + 2.0mg / L BA + 0.2mg / L NAA, white sugar 20 g / L, agar 0.6%, pH 5.6.

[0025] Rooting medium for strong seedlings: 1 / 2MS + 0.1mg / L NAA + 0.1mg / L IBA, activated carbon 0.2%, white sugar 15 g / L, agar 0.5%, pH 5.6.

[0026] 2) Cut the sterilized spare material under sterile conditions into several...

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Abstract

The invention discloses a rapid seedling method of medicinal sarcopyramis bodinieri calluses, and the method comprises the following steps of: (1) disinfecting taken materials; (2) cultivating the disinfected materials on a culture medium; and (3) transplanting and planting seedlings. The method comprises: taking the stem section of the tail end of wild sarcopyramis bodinieri as an explant, inoculating the stem section containing a node to the culture medium for growing under sterile condition, and then breeding into a large amount of sarcopyramis bodinieri seedlings through transplanting a plurality of times. Results indicate that the monthly proliferation rate of the calluses is 4-5 with the most appropriate culture medium and in the appropriate culture environment; each seedling averagely has 5.8 leaves and 4.8 roots; the tissue culture seedlings grow well after being transplanted to a matrix; and the survival rate of the plants in a moth is above 98%. The method provided by the invention is simple in operation and convenient in actual popularization and application.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for rapidly growing seedlings of medicinal spay grass buds. Background technique [0002] Sarcopyramis bodinieri Levl. Et Van is a wild peony plant, which grows under dense forests, damp places or rock crevices in valleys at an altitude of 1000-2450m. "Chinese Materia Medica" records: Styrofoam clears heat and dampness; reduces swelling and detoxification. Mainly heat-toxin blood dysentery; heat-damp diarrhea; cough due to lung heat; conjunctival congestion and swelling pain; vomiting blood; furuncle swollen toxin; trauma redness and swelling; venomous snake bite. It has the best curative effect when used in the folk to treat acute hepatitis (hepatitis A, hepatitis B in the acute phase), and it is also effective for chronic hepatitis and hepatitis B virus carriers. The sales volume is relatively large in Fujian and southern Fujian, and the wild resources are cu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张绪璋汤绍康张丽吴技泉吴雨英
Owner FUJIAN AGRI & FORESTRY UNIV
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