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Hollow microcapsule, acidic or alkaline controlled-release microcapsule and preparation methods thereof

A microencapsulation and alkaline technology, applied in the field of biological preparations, can solve the problems of short-lasting effect and no report of insecticidal protein microencapsulation

Active Publication Date: 2014-07-30
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these studies are mainly aimed at the microencapsulation of Bacillus thuringiensis thallus, and the microencapsulation of insecticidal proteins has not been reported yet.
Moreover, the existing capsule dosage form does not have a long-lasting effect on bacterial protection, and the encapsulated content will be released into the environment as time goes by.

Method used

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  • Hollow microcapsule, acidic or alkaline controlled-release microcapsule and preparation methods thereof
  • Hollow microcapsule, acidic or alkaline controlled-release microcapsule and preparation methods thereof
  • Hollow microcapsule, acidic or alkaline controlled-release microcapsule and preparation methods thereof

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Embodiment Construction

[0045] The present invention will be further described in detail below with reference to the examples, which are not intended to limit the present invention.

[0046] 1 Preparation of empty microcapsules

[0047] 1.1 Preparation of calcium carbonate core

[0048] Prepare 0.2M NaCO 3 solution and added with NaCO 3 The same mass of PSS (sodium polystyrene sulfonate) was mixed.

[0049] Prepare 0.2M CaCl 2 solution.

[0050] The above equivalent amount of NaCO 3 Solution and CaCl 2 Each solution was 100ml, and the mixture was rapidly stirred for 30min.

[0051] Centrifuge, 5000rpm, 10min, remove supernatant. The precipitate was washed with water and centrifuged at 5000 rpm for 10 min. The supernatant was discarded and repeated three times.

[0052] 1.2 Preparation of capsule wall by self-assembly method

[0053] A 1 mg / ml solution of PAH (polyacrylamine hydrochloride) was prepared with 100 mM Tris, and the pH was adjusted to 7.0 with HCl.

[0054] A 1 mg / ml PSS solutio...

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Abstract

The invention relates to a hollow microcapsule, an acidic or alkaline controlled-release microcapsule and preparation methods thereof, belonging to the field of biological agents. The preparation method of the hollow microcapsule comprises the following steps of: preparing a microcapsule through a layer-by-layer self-assembly method by taking calcium carbonate as a core and selecting polyelectrolyte cation and anion with opposite charges under a preparation condition; and then removing the core to obtain the hollow microcapsule. The wall of the hollow microcapsule is formed by alternating PAH / PSS (Polypropylene Amine hydrochloride / Poly Sodium Styrenesulfonate), and the hollow microcapsule can loaded with Cry protoxins of bacillus thuringiensis insecticidal crystal proteins under an acidic condition so as to obtain a microcapsule dosage form with release controlled under an alkaline condition. The microcapsule dosage form keeps the insecticidal activity of the cry protoxins, can assist the cry protoxins to resist the influence of certain environmental factors, also enables the insecticidal proteins to be specifically released in an alkaline environment of an insect midgut and keep stable in a general environment and plays an important role in the actual application of the Bt (Bacillus thuringiensis) insecticidal proteins on pest control.

Description

technical field [0001] The invention relates to the field of biological preparations, in particular to an empty microcapsule, an acidic or alkaline controlled-release microcapsule and a preparation method thereof. Background technique [0002] Bacillus thuringiensis (Bt) is a Gram-positive Agrobacterium belonging to the genus Bacillus, widely distributed in leaves, insect corpses, soil, storage dust, and sewage. It can produce paraspore crystals while forming spores, also known as insecticidal crystal proteins (Insecticidal Crystal Proteins, ICPs), parasitic on Lepidoptera, Diptera, Coleoptera, Hymenoptera, Homoptera insects and animals and plants Worm nematodes have specific poisoning effects, but are harmless to humans and livestock, and do not pollute the environment. They are widely used in agricultural and forestry pest control. [0003] Bacillus thuringiensis insecticidal crystal protein, also known as δ-endotoxin, is a kind of crystal protein with specific insecticid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/02A01N25/28A01N63/02A01P7/04
Inventor 郭淑元杨文慧何康来张杰宋福平
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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