Method for rapid screening of microbial mediums

A technology of microbial culture medium and culture medium, which is applied in the field of rapid screening of microbial culture medium, and can solve the problems of cumbersome operation, time-consuming and labor-intensive experiments, and experimental errors

Inactive Publication Date: 2012-10-24
CHENGDU OLYMVAX BIOPHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This traditional screening method needs to do many times of culture experiments, the operation is cumbersome, time-consuming and laborious, it is not convenient to carry out a large number of medium screening experiments, and it is easy to cause experimental errors

Method used

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  • Method for rapid screening of microbial mediums
  • Method for rapid screening of microbial mediums
  • Method for rapid screening of microbial mediums

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] [Example 1] In this example, Haemophilus influenzae Hib was selected as the strain to be tested, and Hib culture medium with different concentrations of hemin and coenzyme I was screened.

[0075] The method for rapid screening of Haemophilus influenzae culture medium comprises the following steps:

[0076] (1) Prepare equipment and equipment: prepare the equipment and equipment needed for the experiment, including biological safety cabinet, 250μl pipette, 96-well microplate, micro-fermentation air-permeable membrane sealing film, constant temperature culture oscillator and 8-channel full-wavelength enzyme standard instrument;

[0077] It also includes a step to sterilize the equipment used in the experiment before use, which includes the following steps:

[0078] (I) Place the biological safety cabinet, 8-channel full-wavelength microplate reader and constant temperature culture oscillator in a clean environment of class C, and fumigate and sterilize with ozone;

[0...

Embodiment 2

[0098] [Example 2] In this example, Haemophilus influenzae Hib was selected as the strain to be tested, and Hib culture medium with different concentrations of hemin and coenzyme I was screened.

[0099] The method for rapid screening of Haemophilus influenzae culture medium comprises the following steps:

[0100] (1) Prepare equipment and equipment: prepare the equipment and equipment needed for the experiment, including biological safety cabinet, 250μl pipette, 96-well microplate, micro-fermentation air-permeable membrane sealing film, constant temperature culture oscillator and 8-channel full-wavelength enzyme standard instrument;

[0101] It also includes a step to sterilize the equipment used in the experiment before use, which includes the following steps:

[0102] (I) Place the biological safety cabinet, 8-channel full-wavelength microplate reader and constant temperature culture oscillator in a clean environment of class C, and fumigate and sterilize with ozone;

[0...

Embodiment 3

[0122] [Example 3] In this example, Haemophilus influenzae Hib was selected as the strain to be tested, and Hib culture medium with different concentrations of hemin and coenzyme I was screened.

[0123] The method for rapid screening of Haemophilus influenzae culture medium comprises the following steps:

[0124] (1) Prepare equipment and equipment: prepare the equipment and equipment needed for the experiment, including biological safety cabinet, 250μl pipette, 96-well microplate, micro-fermentation air-permeable membrane sealing film, constant temperature culture oscillator and 8-channel full-wavelength enzyme standard instrument;

[0125] It also includes a step to sterilize the equipment used in the experiment before use, which includes the following steps:

[0126] (I) Place the biological safety cabinet, 8-channel full-wavelength microplate reader and constant temperature culture oscillator in a clean environment of class C, and fumigate and sterilize with ozone;

[0...

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Abstract

The invention discloses a method for rapid screening of microbial mediums. The method comprises the following steps: preparing bacterium suspension and mediums to be screened; pipetting the mediums to a microplate; pipetting the bacterium suspension to the microplate to inoculate the bacterium suspension to the mediums; sealing the microplate and carrying out shake cultivation for 8 to 10 h; detecting the light absorption value OD of fermentation broth in each micropore by using a microplate reader at an interval of 1 to 2 h; and drawing a growth curve, carrying out comparison and completing screening. The beneficial effects of the invention are as follows: the bacterium suspension of strains to be detected and the differently formulated mediums to be screened are respectively added into each micropore of the microplate for simultaneous fermentation and culture so as to realize the advantages of simple screening steps, convenient operation, a highly accurate thalli growth curve, highly reliable screening results and a fast screening speed, workload of workers is mitigated, cost for screening mediums is reduced, and the method is applicable to a great number of screening experiments of mediums.

Description

technical field [0001] The invention relates to a method for rapidly screening microorganism culture medium. Background technique [0002] Microbial medium is a mixture of a certain proportion of various nutrients that are artificially prepared for the growth and reproduction of microorganisms and the synthesis of various metabolites. At the same time, the medium also provides microorganisms with the necessary environmental conditions for growth other than nutrients . [0003] The composition of the medium has an important impact on the growth and reproduction of bacteria, the biosynthesis of products, the separation and purification of products, and even the quality and yield of products. Therefore, the formulation components of the medium, the content ratio of each formulation component, and the preparation method of the medium will have a major impact on the growth of microorganisms. Choosing a suitable medium is a crucial step in microbial fermentation production. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02G01N21/31
Inventor 伍长华吴强罗力心陈庚关晓峰杨永华
Owner CHENGDU OLYMVAX BIOPHARM
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