Bacillus pumilus and application thereof

A kind of Bacillus pumilus, the technology of deposit number

Inactive Publication Date: 2012-10-31
CHANGAN UNIV
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  • Abstract
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Problems solved by technology

[0002] The microbial remediation technology of oil-contaminated soil has been greatly developed in foreign countries in the past ten years, but the degradation rate of petroleum hydrocarbon pollutants is slow, resulting in a long duration of the treatment process, which has always been a prominent difficulty in this technology, so looking for High-efficiency petroleum-degrading bacteria are currently a research hotspot in microbial remediation of petroleum-contaminated soils

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  • Bacillus pumilus and application thereof
  • Bacillus pumilus and application thereof

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Embodiment Construction

[0028] Screening of strains:

[0029] Step 1. Enrichment: The soil samples collected from the soil oil pollution 10000mg / kg restoration area of ​​the red clover rhizosphere restoration area about 10cm away from the Shaanxi Provincial Groundwater and Ecological Environment Engineering Research Center Test Site in Weiyang District, Chang'an University, Weishui Campus, Xi'an City were sterile Weigh 10.0g, put it into 100.0mL petroleum / inorganic salt liquid medium, and place it in a constant temperature shaker at 28±1℃, 130r / min for enrichment culture for 7 days; In the inorganic salt liquid medium, enrichment and domestication were carried out 3 times, each time for 7 days; the composition of the petroleum / inorganic salt liquid medium was: NaNO 3 0.15%, (NH 4 ) SO 4 0.15%, K 2 HPO 4 0.1%, MgSO 4 ·7H 2 O0.05%, KCl0.05%, FeSO 4 ·7H 2 O0.001%, CaCl 2 0.0002%, distilled water 1000mL, crude oil (Yanchang Petroleum Dehydrated Crude Oil) 0.5%, the above are mass percentages, me...

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Abstract

The invention discloses a bacillus pumilus 4070 whose preservation number is CGMCC No. 6073. In addition, the invention further discloses an application of the bacillus pumilus in biodegradation of petroleum contaminants. According to the invention, as the bacillus pumilus comes from soil, bacillus pumilus strains very fast becomes a dominant population after entering the soil and occupies a certain ecological niche, so as to effectively promote the biodegradation of petroleum hydrocarbons, optimize a soil microbial system, improve physical characteristics of the soil, enhance biological activity of the soil and recover hardened and degraded soils. The bacillus pumilus disclosed by the invention is a predominant strain screened with crude oil as a unique carbon source; the property of thecrude oil can be changed by the strain in the growing and breeding process, so that components of the crude oil are changed, an effect of emusifying, wetting and dispersing the crude oil is achieved and the petroleum contaminants in the soil can be degraded within a short time.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a bacillus pumilus and its application. Background technique [0002] The microbial remediation technology of oil-contaminated soil has been greatly developed in foreign countries in the past ten years, but the degradation rate of petroleum hydrocarbon pollutants is slow, resulting in a long duration of the treatment process, which has always been a prominent difficulty in this technology, so looking for High-efficiency petroleum-degrading bacteria are currently a research hotspot in the microbial remediation technology of petroleum-contaminated soil. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a Bacillus pumilus that can be used to degrade petroleum pollutants in view of the above-mentioned deficiencies in the prior art. This strain is a dominant strain selected from crude oil as the only carb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10A62D3/02C12R1/07A62D101/20
Inventor 申圆圆王文科李春荣王周峰
Owner CHANGAN UNIV
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