Bacillus mycoides and application thereof for promoting growth of liquidambar formosana

A technology of Bacillus mycoides and sweetgum, applied to Bacillus mycoides and its application field in promoting and promoting the growth of Chinese sweetgum, can solve the problem of no reports of Chinese sweetgum organophosphate-resolving bacteria, and types of organophosphate-resolving bacteria. And the number of different problems, to achieve the effect of excellent strain resources, promote growth and development, good degradation effect

Inactive Publication Date: 2012-11-28
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, studies have found that microorganisms have a strong rhizosphere effect, and the types and numbers of organophosphate-decomposing bacteria vary in different soils, different crop rhizospheres, and different environmental conditions.
[0006] At present, there is no report on the organophosphate-decomposing bacteria of Chinese sweetgum sweetgum at home and abroad

Method used

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  • Bacillus mycoides and application thereof for promoting growth of liquidambar formosana
  • Bacillus mycoides and application thereof for promoting growth of liquidambar formosana
  • Bacillus mycoides and application thereof for promoting growth of liquidambar formosana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Determination of Phosphorus Solubilization Capacity of FXY-WH10 Plate.

[0022] Egg yolk medium: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, distilled water 1000mL, agar 15.0~18.0g, pH 7.0, sterilized, when the temperature drops to about 50℃, add 3~4mL to every 50mL Fresh egg yolk liquid (mix the egg yolk and sterile saline in equal volume), mix well, and pour the plate.

[0023] The FXY-WH10 strain that was activated twice was spot-attached to the yolk plate with a sterilized toothpick, and after incubating at 30°C for 3 days, the diameter of the phosphate zone and the colony diameter was measured, and the ratio of the phosphate zone to the colony diameter was calculated ( figure 1 ).

[0024] From figure 1 It can be seen that after the FXY-WH10 strain is cultured on a yolk plate, it can produce an obvious opaque phosphate ring. After measurement and calculation, the diameter of the phosphate ring is 30.15mm, the diameter of the colony is 14.08mm, and ...

Embodiment 2

[0025] Example 2: Determination of FXY-WH10 Liquid Phosphorus Solubilization Ability.

[0026] Monkina medium: glucose 10.0g, ammonium sulfate 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, magnesium sulfate heptahydrate 0.3g, ferrous sulfate heptahydrate 0.03g, manganese sulfate tetrahydrate 0.03g, eggs Phospholipid 0.4g, calcium carbonate 5.0g, distilled water 1000mL, pH 7.0

[0027] Inoculate the activated FXY-WH10 strain twice into NA medium (beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, distilled water 1000mL, agar 18.0g, pH 7.2~7.4). After incubating at 30°C for 2 days, nothing Rinse the cells with bacteria water to make a bacterial suspension. Take 1 mL of the bacterial suspension in a 100 mL Erlenmeyer flask containing 50 mL of Monkina medium, and use the Monkina medium containing 1 mL of sterile bacterial suspension as a control (CK). Three replicates of each treatment, 30°C, 200r / min shaking culture for 72h, the fermentation broth was centrifuged at 4°C, ...

Embodiment 3

[0029] Example 3: FXY-WH10 greenhouse pot experiment.

[0030] After activating FXY-WH10 for 2~3 times, use an inoculating loop to pick a small amount of bacteria and inoculate it in a 100mL Erlenmeyer flask containing 50mL NB medium (3g beef extract, 10g peptone, 5g sodium chloride, 1000mL distilled water, pH 7.2~7.4) , 30℃, 200r / min shaking culture for 48h. The fermentation broth (4℃, 6000r / min) was centrifuged for 5 minutes, and the bacteria were rinsed with sterile saline for 3 times, and then the bacteria suspension (10 8 cfu / mL) made into bacteria. Chinese Liquidambar formosana (seedling age 60 days) was inoculated, and the same amount of sterile saline was used as the control, and the inoculation amount was 5 mL / plant. 8 replicates for each treatment, placed in a greenhouse for unified management, light is 12h / day, and watered in time.

[0031] The effect of FXY-WH10 strain inoculation for 90 days on the growth of annual Chinese Liquidambar seedlings (see Table 1). From T...

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Abstract

The invention discloses bacillus mycoides FXY-WH10. The bacillus mycoides has been collected in the China Center for Type Culture Collection (CCTCC), with collection number being CCTCC NO:M 2012263 and collection date being July 1, 2012. The invention also discloses application of the bacillus mycoides for promoting growth of liquidambar formosana. The FXY-WH10 strains have stronger lecithin degradation capabilities. The FXY-WH10 strains are inoculated to the liquidambar formosana seedlings and the results show that the strains can obviously promote growth of the liquidambar formosana seedlings. Therefore, the invention provides excellent strain resources for developing liquidambar formosana phosphorus bacterial fertilizers.

Description

Technical field [0001] The invention belongs to the technical field of microbial fertilizers in the field of biological fertilizers, and specifically relates to a Bacillus mycoides and its application in promoting the growth of Liquidambar formosana. Background technique [0002] Chinese sweetgum (Liquidambar formosana) is a large deciduous tree of the Hamameliaceae Chinese sweetgum genus. The tree is tall and straight, with a broad crown, and majestic. The leaves are red in late autumn, arrogant and beautiful. It is beautiful and magnificent. It is a beautiful garden to watch. Tree species and famous autumn tree species in southern my country. Liquidambar formosana is naturally distributed in the area south of the Huai River in the Qinling Mountains, from Sichuan and Guizhou in the west, Guangdong in the south, and Taiwan in the east. The vertical distribution is generally in hills and plains below 1000~1500m above sea level. Liquidambar formosana is very suitable for the estab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/00C12R1/07C09K101/00
Inventor 陈凤毛徐国芳任嘉红张明艳
Owner NANJING FORESTRY UNIV
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