Dendrobium officinale seedling hardening method

A technology for dendrobium candidum and seedling hardening is applied in the field of dendrobium candidium tissue culture seedling hardening, which can solve the problems of high cost, achieve the effects of reducing the cost of hardening seedlings, maintaining water and nutrients, and simplifying the hardening operation

Inactive Publication Date: 2012-12-19
RENHUA XINYU ECOLOGY DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the application of chemical fertilizers or nutrient solutions can shorten the growth period to a certain extent, the cost is relatively high

Method used

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  • Dendrobium officinale seedling hardening method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Selection of tissue-cultured seedlings in bottle: select Dendrobium officinale seedlings with 3-8 roots and seedling height ≥ 5cm, take it out from the bottle gently, clean the attached medium in clear water, and put The seedlings are thinned and dried in the shade. After the root system of the seedlings turns white, they can be planted in the seedling hardening medium after routine disinfection.

[0026] 2. Selection and placement of seedling hardening substrate: first prepare seedling hardening substrate, mix camphor wood chips with a thickness of 0.3cm and gravel with a particle size of 1.5cm according to the mass ratio of 7:3 and stir to form a mixture, and soak it thoroughly with water For camphor wood sawdust, cook the mixture with steam at 100°C for 10 hours, and then sterilize it for later use; take the prepared and sterilized seedling hardening substrate and lay it in a seedling hardening flower pot to a thickness of 12 cm, spray an appropriate amount of wate...

Embodiment 2

[0034] 1. The selection of tissue culture seedling bottle seedling: with embodiment 1.

[0035] 2. Selection and placement of seedling hardening substrate: first prepare seedling hardening substrate, mix camphor wood chips with a thickness of 0.4cm and gravel with a particle size of 2.5cm according to the mass ratio of 6.5:3.5 and stir evenly to form a mixture, and soak it thoroughly with water For camphor wood sawdust, cook the mixture with steam at 110°C for 10 hours, and then sterilize it for later use; take the prepared and sterilized seedling hardening substrate and spread it in a seedling hardening flower pot to a thickness of 12 cm, spray an appropriate amount of water to control the humidity of the substrate at 60%.

[0036] 3. Selection of fertilizer for seedlings: Use organic fertilizer made by mixed fermentation of sheep manure or cow dung, EM biological bacteria and molasses. The fertilizer is applied to the roots of the seedlings. The fertilization frequency is onc...

Embodiment 3

[0043] 1. The selection of tissue culture seedling bottle seedling: with embodiment 1.

[0044] 2. Selection and placement of seedling hardening substrate: first prepare seedling hardening substrate, mix camphor wood chips with a thickness of 0.6cm and gravel with a particle size of 3cm according to the mass ratio of 7.5:2.5 and stir evenly to form a mixture, and soak camphor wood thoroughly with water Sawdust, cook the mixture with steam at 120°C for 10 hours, and then sterilize it for later use; take the prepared and sterilized seedling hardening substrate and spread it in a seedling hardening flower pot to a thickness of 12 cm, spray an appropriate amount of water to control the humidity of the substrate at 60%.

[0045] 3. Selection of fertilizer for seedlings: Use organic fertilizer made by mixed fermentation of sheep manure or cow dung, EM biological bacteria and molasses. The fertilizer is applied to the roots of the seedlings. The fertilization frequency is once every 7...

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Abstract

The invention provides a dendrobium officinale seedling hardening method. The method comprises the following steps of: selecting tissue culturing bottle seedlings; selecting and laying a seedling hardening medium; selecting a seedling hardening fertilizer; planting the seedlings; and managing the seedlings after the planting; and the method is characterized in that the seedling hardening medium is obtained by mixing hardwood chips and broken stones according to a mass ratio of 6.5-7.5:2.5-3.5; and the seedling hardening fertilizer is an organic fertilizer obtained by fermenting wastes of herbivores. The seedling hardening medium used in the method provided by the invention has the advantages of easily obtained raw materials, no need of applying the chemical fertilizer, low seedling hardening cost, tissue culturing seedling hardening survival rate of more than 98%, developed root systems of obtained acclimated seedlings, thick and strong stems, thick and dark green leaves and strong adaptive capacity to environment.

Description

[0001] Field [0002] The invention relates to the technical field of plant cultivation, in particular to a method for hardening tissue-cultured seedlings of Dendrobium officinale, belonging to the technical field of tissue-cultured seedlings of Dendrobium candidum. Background technique [0003] Dendrobium officinale, also known as Dendrobim officinale Kimura et Migo, belongs to Angiosperm, Monocotyledon, Microorder, Orchidaceae, and Dendrobium. It is a precious Chinese medicinal material with high medicinal value and health care function. powerful. Dendrobium officinale naturally grows wild on cliffs tens of meters high. Wild Dendrobium officinale has low natural reproductive capacity, slow growth, and harsh environmental conditions for growth. In recent years, due to the destructive excavation of wild resources of Dendrobium candidum, wild Dendrobium candidum is on the verge of extinction. At present, the state has listed Dendrobium officinale as a rare and endangered spec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01H4/00C05F17/00C05F3/00
CPCY02A40/20Y02W30/40
Inventor 王汉友李细罗
Owner RENHUA XINYU ECOLOGY DEV
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