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Application of Wuling capsule in preparation of medicament for inhibiting proliferation of U251 cell

A U251, cell proliferation technology, applied in the direction of drug combination, antineoplastic drugs, medical raw materials derived from fungi, etc., can solve the problem of no glioblastoma cell proliferation

Inactive Publication Date: 2012-12-19
NANJING ZHENGLIANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Wuling Capsule is the exclusive product of Zhejiang Zuoli Pharmaceutical Co., Ltd., and there is no literature report on its inhibition of glioblastoma (U251) cell proliferation

Method used

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  • Application of Wuling capsule in preparation of medicament for inhibiting proliferation of U251 cell

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0008] Experimental Research Data of Wuling Capsules Inhibiting the Proliferation of U251 Cells

[0009] 1 Experimental materials

[0010] 1.1 Experimental cell lines

[0011] Glioma (U251), from our company's laboratory cell bank, was cultured in DMEM+10%FBS.

[0012] 1.2 Experimental drugs

[0013] Research drug: Wuling capsule, Zhejiang Zuoli Pharmaceutical Co., Ltd., batch number: 20110423

[0014] Liquid storage liquid: Weigh 100 mg of Wuling capsule content, dissolve in 5 ml of absolute ethanol, filter through a 0.2 μm filter, aliquot into 500 μldoff tubes, store at -20°C, and filter absolute ethanol through a 0.2 μm filter for use in the control group use.

[0015] 1.3 Experimental reagents

[0016] DMEM (GIBCO Cat.No.12100-061 Lot.No.758137); Fetal Bovine Serum (Zhejiang Tianhang Biotechnology Co., Ltd. Lot.No.100419); NaHCO3 (Shanghai Jiuyi Chemical Reagent Co., Ltd. Cat.No.11810 -033Lot.No.1088387); Trypsin (AMRESCO lot number: 2010 / 04); EDTA (AMRESCO lot numbe...

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Abstract

The invention discloses application of a Wuling capsule in the preparation of a medicament for inhibiting proliferation of a U251 cell. The Wuling capsule comprises various main components such as Wuling fungus powder, vitamins of VitE, B1, B6, K1 and the like and trace elements of Zn, Fe, Ca and the like, wherein the Wuling fungus powder contains 19 kinds of amino acids, such as adenosine, polysaccharide, sterols, glutamic acid, Y-aminobutyric acid and lysine. The Wuling capsule has the functions of reinforcing the kidney and brain and tranquilizing mind by nourishing the heart and is suitable for treating disharmony of heart and kidney of neurasthenia, insomnia, amnesia, physical and mental fatigue, soreness and weakness of waist and knees, minor veins or myasthenia gravis and the like. The Wuling capsule is orally taken by thee granules each time, with three times each day or follows the advice of doctors; the weight of each granule is 0.33g; and the Wuling capsule is found to have the efficacy of inhibiting the proliferation of the U251 cell.

Description

technical field [0001] The invention relates to a new application of a Chinese patent medicine, in particular to the application of Wuling capsules in the preparation of drugs for inhibiting U251 cell proliferation. Background technique [0002] Glioma, also known as glioma, or glioma for short, is a tumor that occurs in the neuroectoderm, so it is also called neuroectodermal tumor or neuroepithelial tumor. Tumors originate from mesenchymal cells, namely glia, ependyma, choroid plexus epithelium, and nerve parenchymal cells, namely neurons. Most tumors originate from different types of glia, but according to the histogenetic origin and similar biological characteristics, various tumors that occur in the neuroectoderm are generally called gliomas. The course of glioma varies depending on the pathological type and location. The time from the onset of symptoms to seeing a doctor is generally several weeks to several months, and a few can reach several years. The medical histo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/06A61P35/00
Inventor 不公告发明人
Owner NANJING ZHENGLIANG MEDICAL TECH
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