Pretreatment method for alkaloid in Anisodus tanguticus

A technology of alkaloids and anisodamine, which is applied in the field of pretreatment of natural medicines, can solve problems such as selective enrichment of difficult alkaloids, and achieve the effects of ensuring reproducibility and stability, good enrichment effect and high selectivity.

Inactive Publication Date: 2013-01-02
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Application Information

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Problems solved by technology

The traditional solid-phase extraction method is usually carried out in aqueous solution, and there are often some alkaloid compounds with strong hydrophobicity in alkaloids, which are difficult to dissolve in aqueous solution, so the traditional solid-phase extraction method is difficult to achieve this kind of biological Alkaline selective enrichment

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  • Pretreatment method for alkaloid in Anisodus tanguticus
  • Pretreatment method for alkaloid in Anisodus tanguticus
  • Pretreatment method for alkaloid in Anisodus tanguticus

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1: Selective enrichment of alkaloids in Anisodamine

[0028] Alkaloids represented by tropane alkaloids widely exist in anisodamine and have received widespread attention because of their high activity. However, there are a large number of non-alkaloids in the extract of Anisodamine, which greatly interferes with the separation, analysis and preparation of alkaloids. Especially for tropane alkaloids that need to be detected at low wavelengths, the interference of non-alkaloids is very serious. In order to remove non-alkaloids in the extract and enrich alkaloids, especially some low-abundance alkaloids, the pretreatment method of alkaloids is particularly important.

[0029] In order to verify the feasibility of the pretreatment method, representative tropane alkaloids-scopolamine and hyoscyamine standard samples were selected for method validation. Dissolve 10 mg of scopolamine and scopolamine in methanol respectively to prepare 1 mg / ml scopolamine-methanol so...

Embodiment 2

[0037] Embodiment 2: Quantitative analysis of hyoscyamine and hyoscyamine in anisodamine

[0038] Scopolamine and scopolamine are two common alkaloids in Anisodamine, which have received widespread attention due to their high activity and medicinal value. Therefore, the quantitative analysis of scopolamine and scopolamine in anisodamine is of great significance to the quality control of anisodamine. In this implementation case, the alkaloid pretreatment method we developed was used for the quantitative analysis of these two alkaloids in Anisodamine, and a simple and feasible new method was developed for their quantitative analysis.

[0039] In order to verify the feasibility of the pretreatment method for treating scopolamine and scopolamine, before processing the actual samples, it was verified with scopolamine and scopolamine standards. First, 10 mg of scopolamine and scopolamine were dissolved in methanol to prepare 1 mg / ml scopolamine-methanol solution and scopolamine-met...

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Abstract

The invention provides a pretreatment method for alkaloid in Anisodus tanguticus. The method is suitable for sample pretreatment in separation, preparation and qualitative and quantitative operation of alkaloid in Anisodus tanguticus. The method mainly includes pulverizing Anisodus tanguticus medicinal material, adding ethanol for extraction, collecting extracting solution, concentrating to obtain extract, tanking the extract, performing selective enrichment to the alkaloid in crude alkaloid by silica gel-based strong cation exchange (SCX) solid phase extraction (SPE) material to obtain alkaloid enriched segment for use in separation analysis and separation preparation of alkaloid in Anisodus tanguticus. The invention has good selectivity and high alkaloid content in fraction, and can greatly improve separation analysis and separation preparation efficiency of alkaloid in Anisodus tanguticus. The pretreatment method has high repeatability and good operability in operating process, is easy for realizing standardization and industrialization, and has a certain directing significance for selectively obtaining alkaloid enriched fraction from Anisodus tanguticus.

Description

technical field [0001] The invention relates to the pretreatment of natural medicines, in particular to the pretreatment method of alkaloids in anisodamine, mainly relating to the extraction of alkaloids in anisodamine and the SPE process. technical background [0002] The Tibetan medicine Scopolonia is the root of Scopolia tangutica Maxim., a plant of the family Solanaceae. It has antispasmodic, analgesic, and hypnotic effects (Guo Pengju et al., Qinghai Authentic Real Estate Medicinal Materials. Xi'an: Shaanxi Science and Technology Press, 1996) . Mainly produced in Gansu, Qinghai, Tibet, Sichuan, Yunnan and other provinces. Among them, tropane alkaloids represented by scopolamine, hyoscyamine and anisodine are the main active ingredients of anisodamine (Zhou Yunlong, Plant Biology, 2004). Pharmacological studies have shown that the tropane alkaloids in Anisodamine have antispasmodic and analgesic effects, (Xu Jingying et al., Analytical Laboratory, 2009, 12). Due to th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D451/10
Inventor 梁鑫淼龙珍张秀莉王超然郭志谋
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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