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Microbial preparation for rehabilitating phenyl amine polluted water body

A technology of microbial preparations and aniline, applied in the field of microorganisms, can solve problems such as abnormal operation of sewage treatment equipment, and achieve the effects of improving biological treatment efficiency, increasing the amount of treated water, and high activity

Inactive Publication Date: 2014-08-13
朱文霞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many chemical plants have used degradative microorganisms to treat aniline compounds in industrial wastewater, and have achieved remarkable results, but they still cannot fully meet the actual needs. A considerable number of industrial polluting enterprises in our country would rather be punished than invest in wastewater treatment. The operation of the sewage treatment plant is also extremely abnormal

Method used

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  • Microbial preparation for rehabilitating phenyl amine polluted water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A microbial preparation for repairing aniline-polluted water bodies, the active ingredient of the preparation includes the following raw materials in weight percentage:

[0034] Pseudomonas aeruginosa 25%, nitrosative bacteria 15%, Bacillus subtilis 15%, Alcaligenes faecalis 15%, Saccharomyces cerevisiae 10%, Rhodococcus 10%, Bacillus megaterium 5%, Aspergillus niger 5%.

[0035] Among the above microbial preparations:

[0036] The Pseudomonas aeruginosa is preferably Pseudomonas aeruginosa (Pseudomonasaeruginosa) ATCC15442;

[0037] The nitrosative bacteria is preferably Nitrosomonas europaea CCTCC No: M2010002;

[0038] The Bacillus subtilis is preferably Bacillus subtilis (Bacillus subtilis) CGMCC No: 0954;

[0039] The Alcaligenes faecalis is Alcaligenes faecalis ATCC31555;

[0040] The Saccharomyces cerevisiae is preferably Saccharomyces cerevisiae CCTCC No: M208110;

[0041] The rhodococcus is preferably Rhodococcus rhodochrous (Rhodococcus rhodochrous) ATCC1590...

Embodiment 2

[0056] The usage method of the microbial solid preparation obtained in Example 1: drop the solid preparation into the polluted water body of aniline, wherein the weight ratio of the solid preparation and the water body is 1:500, wherein the polluted water body of aniline is artificially prepared, and the initial concentration of aniline is 48.79mg / L, and then shaking culture at room temperature for 24 hours, the concentration of aniline dropped to 0.01mg / L, the degradation rate can reach 99.9%.

Embodiment 3

[0058] The microbial solid preparation prepared in Example 1 is used to treat sewage from a chemical plant. In the sewage, the concentration of COD is 420mg / L, the concentration of dinitrotoluene+trinitrotoluene is 120mg / L, and the concentration of aniline is 58mg / L. Add 20 grams of the preparation prepared in Example 1 to rice sewage each time, once a day, and after 10 days of continuous dosing, the treatment effect detected is as follows: the concentration of effluent COD is 20 mg / L, and the concentration of nitrobenzenes is 1 mg / L or less, the concentration of anilines is 0mg / L.

[0059]

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Abstract

The invention relates to a microbial preparation for treating a phenyl amine polluted water body, and a preparation method and application thereof. The microbial preparation is prepared from the following raw materials in percentage by weight: 25% of Pseudomonas aeruginosa, 15% of nitrococcus, 15% of Bacillus subtilis, 15% of Bacillus foecalis alkaligenes, 10% of Saccharomyces cerevisiae, 10% of Rhodococcus, 5% of Bacillus megaterium and 5% of Aspergillus niger. According to the invention, the strains are matched with each other reasonably, and realize symbiotic coordination without mutual antagonism; and when the microbial preparation is thrown into a wastewater treatment system, nondegradable phenyl amine compounds can be favorably degraded. Thus, the microbial preparation is suitable for industrial sewage treatment, improves the quantity and quality of treated water, lowers the operating cost and promotes the standardized discharge.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a microbial preparation for treating amine-polluted water bodies and a preparation method thereof. Background technique [0002] With the increasing urban population and the continuous development of industry and agriculture, water pollution accidents occur frequently, seriously endangering the health of people and animals and even life-threatening. The composition of industrial sewage is more complex. Aniline is a widely used chemical raw material, which seriously pollutes the environment and endangers human health, and is carcinogenic. Due to the toxicity of aniline compounds to organisms, people have always paid attention to them. In my country, aniline compounds are listed as key pollutants under priority control in the environment, and industrial drainage is strictly managed, and the highest allowable discharge standard has been formulated, with a concent...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/14C02F3/34C12R1/385C12R1/125C12R1/05C12R1/11C12R1/01C12R1/865C12R1/685C02F101/38
Inventor 朱文霞李丽萍
Owner 朱文霞