3 results about "Rhodococcus rhodochrous" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to glyoxal modified polyacrylamide as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, mixing seed solutions of pseudomonas chlororaphis, rhodococcus roseus and phytobacterium plantarum, inoculating into a fermentation culture medium, and culturing to obtain a mixed seed solution; acrylonitrile is fed in a gradient manner, and the reaction is continued to obtain acrylamide; dissolving acrylamide and diallyldimethylammonium chloride in water to obtain a mixed solution, adding an initiator, and heating for reaction to obtain a cationic polyacrylamide aqueous solution; and finally, reacting the cationic polyacrylamide aqueous solution with the glyoxal aqueous solution. According to the preparation method, a microbial fermentation synthesis method is adopted, the acrylamide monomer can be obtained through a one-step fermentation method by utilizing the synergistic effect of pseudomonas chlororaphis, rhodococcus roseus and phytobacterium plantarum, and glyoxal modified polyacrylamide is finally obtained, so that the paper performance can be obviously improved, and the retention rate is increased.

The present application belongs to the field of environmental microbial remediation technology, and particularly relates to a soil remediation microbial composition, a preparation method and application thereof. The soil remediation microbial composition is composed of the following strains: Pseudomonas aeruginosa, Rhodococcus rhodochrous, Bacillus mucilaginosus and Acinetobacter junii; the mass ratio of the Pseudomonas aeruginosa, the Rhodococcus rhodochrous, the Bacillus mucilaginosus and the Acinetobacter junii is 1-3:1-2:0.5-1.5:0.5-1.5, and the total content of the Pseudomonas aeruginosa, the Rhodococcus rhodochrous, the Bacillus mucilaginosus and the Acinetobacter junii is 1x10 9 -1x10 11 CFU. The present application utilizes the metabolism of microorganisms itself, does not introduce chemical reagents, and has no secondary pollution; the biological surfactant has good environmental compatibility, the ecological recovery of soil after remediation is fast, and the degradation efficiency of organic pollutants is high.

The application belongs to the technical field of microorganisms and particularly relates to a glyoxal modified polyacrylamide as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, seed liquids of Pseudomonas chlororaphis, Rhodococcus rhodochrous and Lactobacillus plantarum are mixed and inoculated into a fermentation medium to obtain a mixed seed liquid; secondly, acrylonitrile is gradiently added and continuously reacted to obtain acrylamide; thirdly, the acrylamide and diallyldimethylammonium chloride are dissolved in water to obtain a mixed liquid, an initiator is added, and the mixed liquid is reacted at a high temperature to obtain a cationic polyacrylamide aqueous solution; and finally, the cationic polyacrylamide aqueous solution is reacted with a glyoxal aqueous solution to obtain the glyoxal modified polyacrylamide. The glyoxal modified polyacrylamide is obtained by using a microbial fermentation synthesis method and through the synergistic effect of Pseudomonas chlororaphis, Rhodococcus rhodochrous and Lactobacillus plantarum, so that acrylamide monomers can be obtained by one-step fermentation, and the glyoxal modified polyacrylamide can be finally obtained, which can obviously improve the paper performance and improve the retention rate.