7 results about "Rhodococcus rhodochrous" patented technology

The invention discloses a haloalkane dehalogenase mutant and an application thereof in degradation of 1, 2, 3-tribromopropane, haloalkane dehalogenase DhaA31 coded by dhaA gene of a Rhodococcus rhodococcus NCIMB 13064 strain is modified to obtain the haloalkane dehalogenase mutant F168W / I246C, the haloalkane dehalogenase mutant has high degradation efficiency on haloalkane pollutant 1, 2, 3-tribromopropane, and the haloalkane dehalogenase mutant has high degradation efficiency on haloalkane pollutant 1, 2, 3-tribromopropane under the condition of water phase normal temperature. The degradation rate of the alkyl halide dehalogenase mutant to 10 mM of 1, 2, 3-tribromopropane within 1 h reaches 100%, and the degradation rate of the alkyl halide dehalogenase mutant to 10 mM of 1, 2, 3-tribromopropane reaches 100%. The haloalkane dehalogenase mutant has high stereoselectivity on (R)-2, 3-dibromo-1-propanol, and the e.r. Value of the haloalkane dehalogenase mutant reaches 6: 94. The haloalkane dehalogenase mutant provided by the invention has high catalytic efficiency and high stereoselectivity, is beneficial to recognition and further degradation of downstream enzymes, and has a good application prospect in bioremediation of emerging haloalkane pollutants.

The present invention discloses a haloalkane dehalogenase mutant and its application in degrading 1,2,3-tribromopropane. Rhodococcus rhodochrous NCIMB 13064 strain dhaA The genetically encoded haloalkane dehalogenase DhaA31 was modified to obtain a haloalkane dehalogenase mutant F168W / I246C. The haloalkane dehalogenase mutant has a high degradation efficiency for the haloalkane pollutant 1,2,3-tribromopropane. Under normal temperature conditions in an aqueous phase, the haloalkane dehalogenase mutant has a degradation rate of 100% for 10 mM 1,2,3-tribromopropane within 1 hour; the haloalkane dehalogenase mutant has high stereoselectivity for (R)-2,3-dibromo-1-propanol, with an er value of 6:94. The haloalkane dehalogenase mutant provided by the present invention has high catalytic efficiency and high stereoselectivity, which is conducive to the recognition and further degradation of downstream enzymes, and has good application prospects in the bioremediation of emerging haloalkane pollutants.

The invention discloses a biological complex microbial inoculant for growth of pitaya in the technical field of pitaya planting. The biological complex microbial inoculant comprises a marine bacterium complex liquid, the marine bacterium complex liquid comprises but is not limited to neoascomycetes, curvularia armbillata, sclerochaete hirsutum, mycoderma, Candicola incanus, streptomycete, streptomyces albidoflavus, leuconostoc mesenteroides, rhodococcus purpureus, lactococcus coriolis, solanum betulini, physotheca acuminata, Diapotheca subclavata, Diapotheca cf.nobilis, streptomycete hydride, streptomycete grassland, streptomycete and penicillium jerinckii, and the marine bacterium complex liquid is prepared from the marine bacterium complex liquid, the marine bacterium complex liquid, the marine bacterium complex liquid, the marine bacterium complex liquid, the marine bacterium complex liquid and the marine bacterium complex liquid. According to the scheme, the marine bacterial strain playing a role in promoting growth of pitaya plants is obtained through high-throughput sequencing screening, and the composite bacterial agent prepared by the scheme can promote growth of pitaya and increase the yield.

The invention belongs to the technical field of microorganisms, and particularly relates to glyoxal modified polyacrylamide as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, mixing seed solutions of pseudomonas chlororaphis, rhodococcus roseus and phytobacterium plantarum, inoculating into a fermentation culture medium, and culturing to obtain a mixed seed solution; acrylonitrile is fed in a gradient manner, and the reaction is continued to obtain acrylamide; dissolving acrylamide and diallyldimethylammonium chloride in water to obtain a mixed solution, adding an initiator, and heating for reaction to obtain a cationic polyacrylamide aqueous solution; and finally, reacting the cationic polyacrylamide aqueous solution with the glyoxal aqueous solution. According to the preparation method, a microbial fermentation synthesis method is adopted, the acrylamide monomer can be obtained through a one-step fermentation method by utilizing the synergistic effect of pseudomonas chlororaphis, rhodococcus roseus and phytobacterium plantarum, and glyoxal modified polyacrylamide is finally obtained, so that the paper performance can be obviously improved, and the retention rate is increased.

The invention relates to the technical field of environmental microorganisms, in particular to a mixed bacterial agent for denitrification of cyanogen-containing wastewater. The microbial inoculum is prepared from five strains, namely alcaligenes alcaligenes YH-2C1, sphingosynium CY-10, calcium acetate-acinetobacter TNJ-1, acinetobacter and rhodococcus ruber C1 according to a proportion, the tolerance concentration of the microbial inoculum to CN <-> concentration reaches 40mg / L, and the tolerance concentration to ammonia nitrogen reaches 2000mg / L. The microbial agent for denitrification of the cyanide-containing wastewater, provided by the invention, is mainly applied to the fields of denitrification of high-ammonia-nitrogen wastewater, denitrification of the cyanide-containing wastewater and the like, has the advantages of high removal efficiency, strong endurance capacity, convenience in use and culture and the like, and can quickly remove ammonia nitrogen and total nitrogen in water under the condition of containing high-concentration cyanide.

The present application belongs to the field of environmental microbial remediation technology, and particularly relates to a soil remediation microbial composition, a preparation method and application thereof. The soil remediation microbial composition is composed of the following strains: Pseudomonas aeruginosa, Rhodococcus rhodochrous, Bacillus mucilaginosus and Acinetobacter junii; the mass ratio of the Pseudomonas aeruginosa, the Rhodococcus rhodochrous, the Bacillus mucilaginosus and the Acinetobacter junii is 1-3:1-2:0.5-1.5:0.5-1.5, and the total content of the Pseudomonas aeruginosa, the Rhodococcus rhodochrous, the Bacillus mucilaginosus and the Acinetobacter junii is 1x10 9 -1x10 11 CFU. The present application utilizes the metabolism of microorganisms itself, does not introduce chemical reagents, and has no secondary pollution; the biological surfactant has good environmental compatibility, the ecological recovery of soil after remediation is fast, and the degradation efficiency of organic pollutants is high.

The application belongs to the technical field of microorganisms and particularly relates to a glyoxal modified polyacrylamide as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, seed liquids of Pseudomonas chlororaphis, Rhodococcus rhodochrous and Lactobacillus plantarum are mixed and inoculated into a fermentation medium to obtain a mixed seed liquid; secondly, acrylonitrile is gradiently added and continuously reacted to obtain acrylamide; thirdly, the acrylamide and diallyldimethylammonium chloride are dissolved in water to obtain a mixed liquid, an initiator is added, and the mixed liquid is reacted at a high temperature to obtain a cationic polyacrylamide aqueous solution; and finally, the cationic polyacrylamide aqueous solution is reacted with a glyoxal aqueous solution to obtain the glyoxal modified polyacrylamide. The glyoxal modified polyacrylamide is obtained by using a microbial fermentation synthesis method and through the synergistic effect of Pseudomonas chlororaphis, Rhodococcus rhodochrous and Lactobacillus plantarum, so that acrylamide monomers can be obtained by one-step fermentation, and the glyoxal modified polyacrylamide can be finally obtained, which can obviously improve the paper performance and improve the retention rate.