31 results about "Rhodococcus rhodochrous" patented technology

The invention discloses a method for biodegradation of organic phosphorus pesticide wastewater, wherein the method comprises the following steps: after wastewater is subjected to electrolysis purification, adding a mixture composed of limestone and calcium chloride, putting an oxidizing agent, forming a calcium salt, and filtering to remove the calcium salt; weighing bacillus amyloliquefaciens Ba2015036, bacillus subtilis, rhodococcus rhodochrous strains R-MS6, aspergillus niger strains J15 and aspergillus niger strains J27 according to the proportion, to prepare a biodegradable agent; sending a part of the obtained wastewater into an SBR reactor, staying for 24 h, then putting a proper amount of the biodegradable agent, carrying out closed aeration for 2 d, and then continuously feeding water with the speed progressively increased at the speed rate of 5%; and adding a proper amount of ferrous sulfate into the wastewater treated by the SBR reactor, carrying out a coagulation reaction, and filtering. Through reasonable selection, proportion and culture of the dominant degrading strains, the biodegradation rate is increased, and at the same time, with collaboration of electrolytic purification, oxidizing agent treatment and SBR reactor treatment, the removal rate of organic phosphorus is greatly increased.

The invention relates to a composite fungicide for treating sludge and a sludge treatment method. The composite fungicide is prepared from the following components in parts by weight: 3 to 8 parts of purple sulfur bacteria, 3 to 8 parts of green sulfur bacteria, 3 to 8 parts of rhodopseudomonas, 3 to 8 parts of rhodopseudomonas capsulata, 3 to 8 parts of rhodococcus rhodochrous, 3 to 8 parts of white-rot fungi, 3 to 8 parts of phosphorus-accumulating bacteria, 3 to 8 parts of anaerobic ammonium oxidation bacteria, 3 to 8 parts of lactobacillus plantarum, 3 to 8 parts of streptomycete, 3 to 8 parts of candida utilis, as well as saccharomyces cerevisiae, lactobacillus lactis, lactobacillus acidophilus, aspergillus niger, aspergillus oryzae, bacillus subtilis and bacillus licheniformis.

A microorganism which is Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. A method of culturing the microorganism in a culture medium comprising urea or urea derivative is claimed. A nitrite hydratase obtainable from the microorganism is claimed. Also claimed is a process of preparing an amide from the corresponding nitrite wherein the nitrite is subjected to a hydration reaction in an aqueous medium in the presence of a biocatalyst selected from the group consisting of a microorganism which is a Rhodococcus rhodochrous strain NUMB 41164, a mutant thereof and a nitrite hydratase obtainable from Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. Also claimed is a method of storing the Rhodococcus rhodochrous NUMB 41164.

The invention provides a nitrile degradation biofilm formed genetically engineered bacterium and application of the genetically engineered bacterium in nitrile-containing wastewater treatment, and belongs to the bioengineering technology. The genetically engineered bacterium Bacillus subtilis N4-pHT01-Nitr with the preservation number of CGMCC No.9484 is preserved in the China General Microbiological Culture Collection Center on July 25, 2014. The bacterium is prepared by inserting a nitrilase gene in Rhodococcus rhodochrous BX2 into Bacillus subtilis N4 for expressing by means of gene engineering. The bacterium is capable of generating a biofilm and degrading acetonitrile, the OD570nm value of biofilm formation capacity is 1.45 when the bacterium is culture in synthetic wastewater at 37 DEG C and 60r/min for 48 hours; and the residual concentration of acetonitrile is 23.56mg/L after the bacterium is oscillated and cultured in 800mg/L acetonitrile-containing synthetic wastewater at 37 DEG C and 160rpm for 48 hours. The bacterium has strong acetonitrile impact resistance, acetonitrile in synthetic wastewater is reduced from 800mg/L to 0.32mg/L when the wastewater is output, and the COD value of the synthetic wastewater is reduced from 487mg/L to 16.39mg/L when the wastewater is output. The bacterium provides a novel method to nitrile-containing wastewater treatment by utilizing a biofilm method.

The invention discloses rhodococcus rhodochrous bacterial strain XHRR1 for purifying ammonia in aquatic water. A preservation name of the bacterial strain XHRR1 is rhodococcus rhodochrous XHRR1, and the classification is named as rhodococcus rhodochrous XHRR1, the preservation unit is China Center for Type Culture Collection, the preservation address is Wuhan China, the preservation date is August3, 2017, and the preservation number is CCTCC NO: M 2017437. The bacterial strain XHRR1 has higher purification capacity for the ammonium in the aquatic water, is good in environment adaptability andhas no unfavorable influence on the cultured prawns. The invention also discloses application of the rhodococcus rhodochrous bacterial strain XHRR1 in purifying the ammonia in the aquatic water.

The invention discloses a microorganism combination solid-state fermentation method for reducing the content of butyl isosulfocyanate in rapeseed dregs. Solid-state fermented rapeseed dregs of the combination of three microorganisms of Rhodococcus rhodochrous, Pediococcus pentosaceus and Candida guilliermondii are adopted for reducing the content of the butyl isosulfocyanate in the rapeseed dregs. The method comprises the following steps: preparation and pretreatment of a rapeseed dreg raw material, preparation of a fermented seed solution, inoculation of the three microorganisms in a proportion, solid fermentation, and preparation of a fermented rapeseed dreg finished product. According to the method, the Rhodococcus rhodochrous is firstly used for enabling the rapeseed dregs to be fermented in a solid state, and is combined with Pediococcus pentosaceus and Candida guilliermondii for use; compared with other technical schemes, the method can greatly reduce the content of the butyl isosulfocyanate in the rapeseed dregs obviously; moreover, the fermentation time is shorter, and the efficiency is high.

The invention relates to rhodococcus rhodochrous for expressing mpd genes and a construction method thereof. The rhodococcus rhodochrous R-D3 for expressing the mpd genes can rapidly degrade residues of Dursban (a pesticide) in a water body. The construction method comprises the following steps: 1. extracting the genome DNA of a slightly acidophilic stenotrophomonas G1 bacterial strain; 2. carrying out amplification to obtain the PCR (polymerase chain reaction) product of the G1 bacterial strain; 3.connecting the PCR product of the G1 strain and pET-28a (+), an escherichia coli plasmid and constructing pET-C, a sequencing recombinant plasmid; and 4. constructing pDA71-C, an expressive recombinant plasmid, constructing an electrically transformed rhodococcus rhodochrous R-D3 bacterial strain of pDA71-C, an expressive recombinant plasmid to obtain the rhodococcus rhodochrous R-D3 for expressing the mpd genes.

The invention discloses a rhodococcus rhodococcus capable of efficiently degrading cyhalothrin and an application thereof. The present invention takes microbial resources in the traditional fermentation process as the research object, screens and obtains the degrading bacteria for cyhalothrin that is used more in the planting process of tobacco and other crops, has a large residual amount, and has a long degradation cycle. The degrading bacteria is rose red The Rhodococcus rhodochrous L5 strain, derived from Fenjiu Daqu, has high safety and can efficiently degrade cyhalothrin at a concentration of 5 mg/kg, and has a certain effect on the degradation of low-concentration cyhalothrin pesticide residues in crops such as tobacco leaves. The application prospect provides a safe and effective biodegradation way to improve the quality and safety of tobacco products and solve the problem of pesticide residues in tobacco leaves and other crops.

A strain of Rhodococcus rhodochrous in which a gene coding at least part of a nitrile hydratase enzyme or any gene coding a protein involved in the transcription, translation or formation of at least part of the nitrile hydratase enzyme has been deactivated or rendered ineffective or a strain of Rhodococcus rhodochrous cultured under condition wherein the nitrile hydratase enzyme is been inhibited.