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Microorganism combination solid-state fermentation method for reducing content of butyl isosulfocyanate in rapeseed dregs

An isothiocyanate, solid-state fermentation technology, applied in microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of long fermentation time, excessive fermentation time, complex fermentation process, etc., and reach the fermentation time. Short, high efficiency, the effect of reducing isothiocyanate content

Inactive Publication Date: 2014-04-09
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a few reports have considered the effect of reducing isothiocyanate and / or oxazolidinethione while mainly targeting the content of glucosinolate, the strains selected in these reports are almost similar and lack novelty. nature, and the fermentation process is relatively complicated, and the fermentation time is generally long, which is not suitable for large-scale production
[0005] At present, based on the fact that there are many strains available for detoxification by microbial fermentation of rapeseed meal, but few strains with good detoxification effect; some parameters in the detoxification process of microbial fermentation of rapeseed meal need to be further optimized, such as fermentation time is too long, etc. ; and in the detoxification of rapeseed meal by microbial fermentation, the report specifically targeting isothiocyanate reduction has not been reported

Method used

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  • Microorganism combination solid-state fermentation method for reducing content of butyl isosulfocyanate in rapeseed dregs
  • Microorganism combination solid-state fermentation method for reducing content of butyl isosulfocyanate in rapeseed dregs
  • Microorganism combination solid-state fermentation method for reducing content of butyl isosulfocyanate in rapeseed dregs

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Embodiment 1

[0016] Embodiment one (laboratory)

[0017] A. Preparation and pretreatment of rapeseed meal raw materials: Rapeseed meal raw materials were purchased from grain, oil and food processing enterprises, and were crushed before sterilization; 50 g of rapeseed meal raw materials were weighed, placed in a 250 mL Erlenmeyer flask, and Add 25mL of water to the Erlenmeyer flask, mix well; adjust the pH to 7.0±0.5, mix well, seal, and sterilize by high pressure steam at 121℃ for 15min;

[0018] B. Preparation of fermented seed liquid: adopt asparagine-glucose medium, lactic acid bacteria medium and potato-glucose-agar medium, three kinds of medium are all liquid medium, adopt shaking flask culture method to be used for cultivating purple red respectively coccus, Pediococcus pentosacea and Pichia montagnis; the medium was sterilized by high-pressure steam at 121°C for 15 minutes, cooled to room temperature, and then inoculated with the corresponding selected strains; The culture tempera...

Embodiment 2

[0024] Embodiment two (pilot test)

[0025] A. Preparation and pretreatment of rapeseed meal raw materials: crush the rapeseed meal raw materials with a pulverizer, weigh 100kg of raw materials, add 50L of water, and mix well; then adjust the pH to 7.0±0.5, mix well, subpackage, seal, Sterilize by high-pressure steam at 121°C for 15 minutes;

[0026] B. Preparation of fermented seed liquid: adopt asparagine-glucose medium, lactic acid bacteria medium and potato-glucose medium, all of which are liquid medium, and use the shake flask culture method to cultivate Rhodococcus rhodochrous and Pediococcus pentosacea respectively and Pichia monteoris; the culture medium was sterilized by high-pressure steam at 121°C for 15 minutes and then cooled to room temperature, and then inoculated with the corresponding selected strains; the culture temperature of Rhodococcus rhodococcus, Pediococcus pentosacea and Pichia mongoliana 28°C, 37°C, and 25-28°C respectively, the shaker speed is 190±...

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Abstract

The invention discloses a microorganism combination solid-state fermentation method for reducing the content of butyl isosulfocyanate in rapeseed dregs. Solid-state fermented rapeseed dregs of the combination of three microorganisms of Rhodococcus rhodochrous, Pediococcus pentosaceus and Candida guilliermondii are adopted for reducing the content of the butyl isosulfocyanate in the rapeseed dregs. The method comprises the following steps: preparation and pretreatment of a rapeseed dreg raw material, preparation of a fermented seed solution, inoculation of the three microorganisms in a proportion, solid fermentation, and preparation of a fermented rapeseed dreg finished product. According to the method, the Rhodococcus rhodochrous is firstly used for enabling the rapeseed dregs to be fermented in a solid state, and is combined with Pediococcus pentosaceus and Candida guilliermondii for use; compared with other technical schemes, the method can greatly reduce the content of the butyl isosulfocyanate in the rapeseed dregs obviously; moreover, the fermentation time is shorter, and the efficiency is high.

Description

technical field [0001] The invention relates to a fermentation method for removing toxic substances from rapeseed dregs, in particular to a microbial combination solid-state fermentation method for reducing the content of isothiocyanate in rapeseed dregs. Background technique [0002] Rapeseed meal is a plant-based protein feed with high nutritional value. Its nutritional value is second only to soybean meal, but it has a price advantage over soybean meal. The crude protein content of rapeseed meal is between 35% and 45%, which is slightly lower than that of soybean meal, but its amino acid composition is slightly better than that of soybean meal, and its vitamin and mineral content is relatively rich. It is a kind of protein feed resource with great application potential . However, rapeseed meal contains a variety of anti-nutritional factors, such as glucosinolates, erucic acid, tannins, phytic acid, etc., especially glucosinolates and their degradation products, which gre...

Claims

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Application Information

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IPC IPC(8): A23K1/00C12N1/20C12N1/16C12R1/01C12R1/72
Inventor 田刚余冰陈代文王乐成
Owner SICHUAN AGRI UNIV
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