Microorganism combination solid-state fermentation method for reducing content of butyl isosulfocyanate in rapeseed dregs
A technology of isothiocyanate and solid-state fermentation, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of long fermentation time, too long fermentation time, complicated fermentation process, etc., and reach the goal of fermentation time Short, less isothiocyanate content, high efficiency effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0016] Embodiment one (laboratory)
[0017] A. Preparation and pretreatment of rapeseed meal raw materials: Rapeseed meal raw materials were purchased from grain, oil and food processing enterprises, and were crushed before sterilization; 50 g of rapeseed meal raw materials were weighed, placed in a 250 mL Erlenmeyer flask, and Add 25mL of water to the Erlenmeyer flask, mix well; adjust the pH to 7.0±0.5, mix well, seal, and sterilize by high pressure steam at 121℃ for 15min;
[0018] B. Preparation of fermented seed liquid: adopt asparagine-glucose medium, lactic acid bacteria medium and potato-glucose-agar medium, three kinds of medium are all liquid medium, adopt shaking flask culture method to be used for cultivating purple red respectively coccus, Pediococcus pentosaceae and Candida quaternium; the medium was sterilized by high-pressure steam at 121°C for 15 minutes, cooled to room temperature, and then inoculated with the corresponding selected strains; The culture temp...
Embodiment 2
[0024] Embodiment two (pilot test)
[0025] A. Preparation and pretreatment of rapeseed meal raw materials: crush the rapeseed meal raw materials with a pulverizer, weigh 100kg of raw materials, add 50L of water, and mix well; then adjust the pH to 7.0±0.5, mix well, subpackage, seal, Sterilize by high-pressure steam at 121°C for 15 minutes;
[0026] B. Preparation of fermented seed liquid: adopt asparagine-glucose medium, lactic acid bacteria medium and potato-glucose medium, all of which are liquid medium, and use the shake flask culture method to cultivate Rhodococcus rhodochrous and Pediococcus pentosacea respectively and Candida quaternidae; the culture medium was sterilized by high-pressure steam at 121°C for 15 minutes and then cooled to room temperature, and then inoculated with the corresponding selected strains; the culture temperature of Rhodococcus rhodococcus, Pediococcus pentosacea and Candida quindida 28°C, 37°C, and 25-28°C respectively, the shaker speed is 19...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com