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A strain of Rhodococcus rhodochrous and its method for producing nicotinamide

A technology of Rhodococcus erythrococcus and nicotinamide, applied in the biological field, can solve the problems of high culture cost and poor genetic stability of strains, and achieve the effects of low culture cost, high enzyme activity and good genetic stability

Active Publication Date: 2022-07-12
山东昆达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also some studies devoted to the expression of enzyme genes using Escherichia coli or Bacillus subtilis as hosts. This method often requires the addition of inducers or antibiotics during microbial culture, and the cost of culture is high, and the genetic stability of the strain is poor.

Method used

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  • A strain of Rhodococcus rhodochrous and its method for producing nicotinamide
  • A strain of Rhodococcus rhodochrous and its method for producing nicotinamide

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1: Rhodococcus rhodochrous strain shake flask culture and transformation

[0022] The shake flask medium was prepared, and the formula was composed of: glucose 1.0%, yeast extract 0.5%, dipotassium hydrogen phosphate 0.2%, magnesium sulfate 0.2%, potassium dihydrogen phosphate 0.2%, monosodium glutamate 0.5%, ammonium sulfate 0.2%. Dissolve with water at 60°C, cool to room temperature and adjust the pH to 7.0, then measure 50ml into a 250ml conical flask, and sterilize at 121°C for 30min for later use. Use an inoculation shovel to excavate an appropriate amount of bacterial mud into the prepared liquid medium, place it on a constant temperature shaker at 35°C, and cultivate at 200rpm for 60h.

[0023] Take 100ml of fermentation broth and separate it with a centrifuge, remove the components of the medium, resuspend the bacterial slurry with pure water to wash the bacterial cells, centrifuge the bacterial cells again, add pure water to suspend the cells to restore...

Embodiment 2

[0026] Embodiment 2: Rhodococcus rhodochrous fermentation culture and reactor transformation

[0027] A medium containing 60% of the volume of the seed tank is added to the seed tank, and the medium includes the following composition in mass percentage: 0.5% yeast extract, 1.0% glucose, 0.3% sodium chloride, 0.1% magnesium sulfate heptahydrate, 0.1% % potassium dihydrogen phosphate, 0.1% dipotassium hydrogen phosphate, 0.1% defoamer; adjust the pH to 7.0 with sodium hydroxide; sterilize at about 121 °C for 28 minutes, and insert the bacteria when the temperature drops to 35 °C. Under the temperature, sterile air was continuously introduced to maintain the tank pressure, and the culture was stirred for 24 hours.

[0028] 60% of the volume of the fermenter is added to the fermenter, and the medium includes the following composition in mass percentage: 0.7% yeast extract, 1.0% glucose, 2% corn steep liquor, 0.2% ammonium sulfate, 0.1% heptahydrate Magnesium sulfate, 0.1% potassi...

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Abstract

The invention belongs to the field of biotechnology, and in particular relates to a strain of Rhodococcus rhodochrous and a method for producing nicotinamide; including steps such as preparation of a Rhodococcus rhodochrous catalyzer, hydration reaction and the like, using nitrile hydratase produced by fermentation of Rhodococcus rhodochrous as a catalyst, using pure Water is used as a reaction system, and 3-cyanopyridine is catalyzed and converted into nicotinamide by means of flow addition or batch addition; the nitrile hydratase-producing Rhodococcus rhodochrous bacterium provided by the invention has good genetic stability, low culture cost, and produces nitrile The hydratase has high enzymatic activity, and this strain can be directly used to convert 3-cyanopyridine into nicotinamide. During the catalytic reaction, pure water is used as the reaction system, and the final reaction concentration of the reaction solution can reach more than 400g / L, avoiding the introduction of exogenous impurities, and at the same time, it is beneficial to the extraction and separation operation, and the industrial application has advantages.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to a strain of Rhodococcus rhodochrous and a method for producing nicotinamide. Background technique [0002] Nicotinamide (nicotinamide; niacinamide), also known as nicotinamide, vitamin B3, vitamin PP, is a white crystalline powder; odorless or almost odorless, bitter in taste; slightly hygroscopic. Soluble in water or ethanol, soluble in glycerol. The clinical drug is vitamin B group, which is used for the prevention and treatment of pellagra, stomatitis, glossitis and other diseases. It is an essential nutrient for mammals, and its water solubility is better than that of niacin. Niacinamide and niacin are common in most situations, and niacin also produces niacinamide in animals. Niacinamide can be used as a nutritional additive in cosmetics. In addition, it is also used in food and feed additives. The market prospect is very broad. [0003] The production of nicoti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/12C12N1/20C12R1/01
CPCC12P17/12C12N1/20
Inventor 李自云邵树强
Owner 山东昆达生物科技有限公司
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