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Rhodococcus jj-3 and its application in degrading acrylic acid

A technology of Rhodococcus rhodochrous and JJ-3 is applied in the field of biological treatment of environmental pollutants, which can solve the problems of difficult control, high operation cost, secondary pollution of heavy metal catalysts, etc.

Active Publication Date: 2022-03-18
ZHEJIANG UNIV OF TECH
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AI Technical Summary

Problems solved by technology

[0004] Catalytic wet oxidation treatment of acrylic acid wastewater has the advantages of high removal efficiency, low energy consumption, and less secondary pollution. It is a relatively clean treatment technology. However, there are problems such as high operating costs, difficult control, and secondary pollution of heavy metal catalysts; The treatment of acrylic acid wastewater by critical water oxidation has the advantages of short reaction time, complete removal of pollutants, small footprint, clean and environmental protection, etc. However, high concentration of dissolved oxygen, equipment corrosion and inorganic salt deposition caused by high temperature and high pressure are the main problems faced by this technology. Problem: Fiber adsorption method, ion exchange fiber method, Fen-ton oxidation method, and photoelectron wave decomposition method to degrade acrylic acid wastewater all have the characteristics of mild reaction conditions, fast reaction speed and high removal efficiency, but due to the lack of in-depth research at present, And the processing cost is high, it has not been applied to the field of actual engineering processing
The use of biological methods to treat acrylic acid wastewater has low energy consumption, mild reaction conditions and high efficiency, which is more in line with the national strategy of energy conservation, emission reduction and sustainable development. It is of great significance to screen available bacterial strains for acrylic acid degradation and carry out biodegradation of acrylic acid

Method used

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  • Rhodococcus jj-3 and its application in degrading acrylic acid
  • Rhodococcus jj-3 and its application in degrading acrylic acid
  • Rhodococcus jj-3 and its application in degrading acrylic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Isolation, purification and identification of RhodococcusruberJJ-3

[0028] 1. Isolation and purification of Rhodococcusruber JJ-3

[0029] RhodococcusruberJJ-3 is screened from the sludge of the wastewater treatment pond of Zhejiang Satellite Energy Co., Ltd. The specific steps are as follows:

[0030] Acrylic acid liquid selection medium, the preparation method is as follows: NH 4 Cl 540mg·L -1 , K 2 HPO 4 1000mg·L-1 , KH 2 PO 4 1000mg·L -1 , NaCl 500mg·L -1 , yeast extract powder 200mg·L -1 , acrylic acid 1000mg·L -1 , MgSO 4 ·7H 2 O 16mg·L -1 , CaCl 2 4mg·L -1 , CuSO 4 4mg·L -1 , FeSO 4 4mg·L -1 , MnSO 4 4mg·L -1 , the solvent is water, the pH value is 7.0, and it is sterilized at 121° C. for 20 minutes.

[0031] Acrylic solid selection medium: NH 4 Cl 540mg·L -1 , K 2 HPO 4 1000mg·L -1 , KH 2 PO 4 1000mg·L -1 , NaCl 500mg·L -1 , yeast extract powder 200mg·L -1 , peptone 400mg·L -1 , acrylic acid 1000mg·L -1 , MgSO ...

Embodiment 2

[0045] Embodiment 2 Rhodococcus erythrococcus JJ-3 fermented culture fluid

[0046] (1) Incline cultivation

[0047] Inoculate Rhodococcus Erythrococcus JJ-3 into the slant medium and culture at 30°C for 3 days to obtain the bacterial slant; the final concentration of the slant medium consists of: NH 4 Cl 540mg·L -1 , K 2 HPO 4 1000mg·L -1 , KH 2 PO 4 1000mg·L -1 , NaCl 500mg·L -1 , yeast extract powder 200mg·L -1 , peptone 400mg·L -1 , acrylic acid 1000mg·L -1 , MgSO 4 ·7H 2 O 16mg·L -1 , CaCl 2 4mg·L -1 , CuSO 4 4mg·L -1 , FeSO 4 4mg·L -1 , MnSO 4 4mg·L -1 , the solvent is water, the pH value is 7.0, and 18g·L -1 agar.

[0048] (2) Seed culture: Pick colonies from the slant of the bacteria and inoculate them into LB medium, cultivate at 30°C for 12 hours to obtain seed liquid; the final concentration of the LB medium is composed of: NaCl 10g L -1 , yeast extract powder 5g·L -1 , peptone 10g·L -1 , the solvent is water, the pH value is 7.0;

[00...

Embodiment 3

[0050] Example 3: RhodococcusruberJJ-3 acrylic acid degradation performance detection

[0051] 1. Investigate the performance of Rhodococcus Erythrococcus JJ-3 in degrading acrylic acid under different initial pH conditions

[0052] The experiment of degrading acrylic acid by Rhodococcus rubrum JJ-3 at different initial pHs found that pH = 7.0 is the optimum pH, and the degradation rate is the highest at this time. The specific implementation steps are as follows:

[0053] With acrylic acid as carbon source (adding concentration 1000mg L -1 ), according to volume concentration 5% inoculum size will OD 600 The 0.8 containing bacteria suspension (prepared by the method of embodiment 2) is inoculated in the acrylic acid liquid selection medium (composition is the same as embodiment 2) of different pH values ​​(pH values ​​are respectively 5.0, 6.0, 7.0, 8.0, 9.0, 10.0), 35°C, 160rpm shaking culture for 16 hours to obtain the culture solution, and use high performance liquid chr...

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Abstract

The present invention discloses a new bacterial strain—Rhodococcus ruber JJ‑3 and its application for degrading acrylic acid. The Rhodococcus ruber JJ‑3 is preserved in the China Center for Typical Culture Collection, address: Wuhan University, Wuhan, China, Postal code: 430072, deposit number: CCTCC NO: M 2019546, date of deposit: July 12, 2019; the invention provides a Rhodococcus erythrococcus JJ‑3 with acrylic acid degradation performance and its application in degrading acrylic acid wastewater. Within 16 hours, the initial concentration is 1000mg·L ‑1 The degradation rate of acrylic acid reached 100%. The discovery of the degrading bacteria is of great significance for the efficient purification of acrylic acid in industrial wastewater.

Description

(1) Technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and in particular relates to a new bacterial strain-Rhodococcus ruber JJ-3 (Rhodococcus ruber JJ-3) with the ability to degrade acrylic acid and its application. (2) Background technology [0002] Acrylic acid is the simplest unsaturated carboxylic acid, consisting of a vinyl group and a carboxyl group, and is a vinyl monomer with a very fast polymerization rate. Acrylic acid is a colorless liquid with a characteristic pungent odor. It is miscible with water, alcohol, ether and chloroform and is prepared from propylene. Acrylic acid is an important organic synthetic raw material and synthetic resin monomer, most of which are used to manufacture acrylates such as methyl acrylate, ethyl ester, butyl ester, and hydroxyethyl ester. Acrylic acid and acrylate can be homopolymerized and copolymerized, and its polymers are used in the production processes of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/34
CPCC12N1/20C02F3/34C02F2101/34C12R2001/01C12N1/205
Inventor 陈浚戴璐瑶胡利勇何金佳张士汉
Owner ZHEJIANG UNIV OF TECH
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