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Rhodococcus rhodochrous bacterial strain XHRR1 for purifying ammonia in aquatic water and application thereof

A technology of Rhodococcus rhodococcus and breeding water, which is applied in the direction of bacteria, water pollutants, chemical instruments and methods, etc., can solve the problems of ignoring the specific actual needs of breeding organisms and production safety, high efficiency and sustainable development, and achieve good results Environmental adaptability, reduced water body replacement, good application effect

Active Publication Date: 2018-03-23
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Instead of simply applying the relevant details in sewage treatment engineering technology, ignoring the specific actual needs of breeding organisms and production for safe, efficient and sustainable development
At present, there is no research report on the use of Rhodococcus rhodochrous to purify ammonia in aquaculture water.

Method used

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  • Rhodococcus rhodochrous bacterial strain XHRR1 for purifying ammonia in aquatic water and application thereof
  • Rhodococcus rhodochrous bacterial strain XHRR1 for purifying ammonia in aquatic water and application thereof
  • Rhodococcus rhodochrous bacterial strain XHRR1 for purifying ammonia in aquatic water and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Screening and cultivation of Rhodococcus rhodococcus XHRR1 for purifying ammonia in aquaculture water

[0025] 1. Material preparation

[0026] 1.1. Bacterial source

[0027] Water samples from 50 to 75 days of intensive prawn culture ponds were collected, and photosynthetic bacteria medium plates were used for isolation and culture.

[0028] 1.2. Medium

[0029] (1) Photosynthetic bacteria liquid medium: CH 3 COONa: 1g, yeast extract: 1g, MgSO 4 ·7H 2 O: 0.4g, NaCl: 0.1g, CaCl 2 2H 2 O: 0.05g, NaHCO 3 : 0.3g, KH 2 PO 4 : 1g, trace element solution 1mL, the above drugs were dissolved in distilled water, and fixed to 1000mL, pH7.0.

[0030] Trace element solution: EDTA: 2.5g, ZnSO 4 ·7H 2 O: 10.95g, MnSO 4 ·H 2 O: 1.54g, CuS0 4 ·5H 2 O: 0.39g, CoCl 2 ·6H 2 O: 0.2g, FeSO 4 ·7H 2 O: 7g, the above drugs were dissolved in distilled water, and fixed to 1000ml, pH7.0.

[0031] (2) Photosynthetic bacteria solid plate culture medium: add agar powde...

Embodiment 2

[0035] Example 2 Identification of Rhodococcus rhodococcus XHRR1 for purifying ammonia in aquaculture water

[0036] The present invention identifies the 16S rDNA molecule of Rhodococcus rhodococcus rose XHRR1 for purifying ammonia in cultured water, and determines the species of the strain from the molecular level, combined with the analysis of the morphological characteristics of the bacteria and the physiological and biochemical characteristics. 16S rDNA sequence analysis mainly follows the following steps:

[0037] 1. Extraction of bacterial genomic DNA:

[0038] (1) Use a sterile toothpick to pick a single colony and inoculate it in the expansion medium for cultivation;

[0039] (2) Take 1.5 mL of bacterial culture solution, centrifuge at 10,000 rpm (11,500 g) for 1 minute, and suck up the supernatant as much as possible;

[0040] (3) Add 200 μL buffer GA to the cell pellet, shake until the cell is completely suspended, add 180 μL lysozyme with a final concentration of ...

Embodiment 3

[0065]Example 3 Small-scale application of Rhodococcus rhodococcus XHRR1 for purifying ammonia in aquaculture water

[0066] 1. Growth of the strain

[0067] The strain Rhodococcus rhodococcus XHRR1 that embodiment 1 obtains is pressed 10 6 CFU / mL was inoculated into sterilized culture pond water, and the bacterial concentration was stable at 10 during 2 to 5 days. 8 CFU / mL quantity level, the growth curve of strain XHRR1 is as follows figure 1 shown in .

[0068] 2. The removal effect of the strain on ammonia in different salinity aquaculture water

[0069] The sterilized aquaculture pond water (water body salinity is 25) is used as the basis for testing the water body, with NH 4 Cl adjusts the concentration of ammonia in the water body to about 24mg / L, and adjusts the salinity of the water body to 5, 15, 25, 35, 45 with distilled water and sea salt. The strain Rhodococcus rhodococcus XHRR1 that embodiment 1 obtains is pressed 10 6 CFU / mL was inoculated into the experim...

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Abstract

The invention discloses rhodococcus rhodochrous bacterial strain XHRR1 for purifying ammonia in aquatic water. A preservation name of the bacterial strain XHRR1 is rhodococcus rhodochrous XHRR1, and the classification is named as rhodococcus rhodochrous XHRR1, the preservation unit is China Center for Type Culture Collection, the preservation address is Wuhan China, the preservation date is August3, 2017, and the preservation number is CCTCC NO: M 2017437. The bacterial strain XHRR1 has higher purification capacity for the ammonium in the aquatic water, is good in environment adaptability andhas no unfavorable influence on the cultured prawns. The invention also discloses application of the rhodococcus rhodochrous bacterial strain XHRR1 in purifying the ammonia in the aquatic water.

Description

technical field [0001] The invention belongs to the technical field of rhodococcus rhodococcus, and in particular relates to a rhodococcus rhodococcus strain XHRR1 for purifying ammonia in aquaculture water and an application thereof. Background technique [0002] In the high-density intensive aquaculture model, the efficient control of the water environment is one of the key technical links in the entire production process. Ammonia nitrogen has serious toxic effects on aquatic animals, and it is easy to accumulate in large quantities in high-density aquaculture water (Zhou Ping 2013), so it is extremely important to effectively control the content of harmful nitrogen in water (Liu Xingguo 2011). At present, the common treatment methods at home and abroad mainly include physical, chemical and biological methods. The physical method uses the adsorption capacity of solids to purify ammonia nitrogen. The commonly used materials are mainly zeolite powder, dolomite powder, coral...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/20C02F101/16
CPCC02F3/34C02F2101/16C02F2103/20C12N1/205C12R2001/01
Inventor 曹煜成文国樑徐煜杨铿胡晓娟徐武杰李卓佳苏浩昌许云娜田雅洁
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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