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A strain of Rhodococcus rhodochrous that efficiently degrades cyhalothrin and its application

A technology of Rhodococcus roseus and cyhalothrin, which is applied in the field of microbiology, can solve problems such as strain safety doubts and application restrictions, and achieve high safety and improve quality and safety.

Active Publication Date: 2021-06-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, pesticide-degrading bacteria are mainly screened from soil and water sludge with serious pesticide pollution, and are mainly used to solve pesticide pollution in soil and water. Most of the screened strains are Pseudomonas and Klebsiella, etc. , the safety of the strain is questioned, and its application is also subject to certain restrictions

Method used

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  • A strain of Rhodococcus rhodochrous that efficiently degrades cyhalothrin and its application
  • A strain of Rhodococcus rhodochrous that efficiently degrades cyhalothrin and its application
  • A strain of Rhodococcus rhodochrous that efficiently degrades cyhalothrin and its application

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Experimental program
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Effect test

Embodiment 1

[0042] Isolation, identification and preservation of embodiment 1, L5 bacterial strain

[0043] 1. Enrichment, domestication, separation and purification of pesticide-degrading bacteria

[0044] 1. Enrichment and domestication of pesticide-degrading bacteria

[0045] Strains were screened from fresh Daqu from Fenjiu Factory. The specific steps are as follows: weigh 10g of Fenjiu Daqu in a 90mL saline flask, incubate at 30°C and 180rpm for 40min, then transfer 1mL to 9mL MSM medium containing 50mg / kg cyhalothrin, and incubate at 30°C , 180rpm for 7 days, then transfer 1mL to 9mL MSM medium containing 100mg / kg cyhalothrin, culture at 30℃, 180rpm for 7d, continue to transfer according to the above operation, and gradually increase cyhalothrin The concentration to 200-400mg / kg.

[0046] 2. Isolation and purification of pesticide-degrading bacteria

[0047] The enrichment and domestication experiment of degrading bacteria lasted 28 days in the early stage, and in the later stag...

Embodiment 2

[0056] Embodiment 2, the application of Rhodococcus rhodochrous L5 in degrading cyhalothrin

[0057] 1. Preparation of bacterial suspension

[0058] Pick Rhodococcus rhodochrous L5 from the LB solid medium and transfer it to the vial containing the LB broth medium, and cultivate it on a shaker (150rpm) at 30°C until the colony OD600=1.0±0.1; then The bacterial solution was centrifuged at 5000×g for 5 minutes, the supernatant was discarded, the bacterial precipitate was washed with MSM liquid medium and mixed evenly. After repeated washing twice, the MSM liquid medium was added, and the vortex oscillator was used to shake and resuspend. The bacteria were mixed thoroughly to prepare a bacterial suspension, and the bacterial concentration of the bacterial suspension was 10 7 cfu / mL.

[0059] 2. Degradation of cyhalothrin experiment

[0060] 1. According to the tobacco leaf pesticide residue standard YQ 50-2014 of China National Tobacco Corporation, the maximum limit standard f...

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Abstract

The invention discloses a rhodococcus rhodococcus capable of efficiently degrading cyhalothrin and an application thereof. The present invention takes microbial resources in the traditional fermentation process as the research object, screens and obtains the degrading bacteria for cyhalothrin that is used more in the planting process of tobacco and other crops, has a large residual amount, and has a long degradation cycle. The degrading bacteria is rose red The Rhodococcus rhodochrous L5 strain, derived from Fenjiu Daqu, has high safety and can efficiently degrade cyhalothrin at a concentration of 5 mg / kg, and has a certain effect on the degradation of low-concentration cyhalothrin pesticide residues in crops such as tobacco leaves. The application prospect provides a safe and effective biodegradation way to improve the quality and safety of tobacco products and solve the problem of pesticide residues in tobacco leaves and other crops.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to the function and characteristics of a rhodococcus rhodococcus strain that efficiently degrades cyhalothrin. Background technique [0002] With the centralized development of agriculture, pesticides are widely used around the world. On the one hand, the use of pesticides can increase the yield of crops; on the other hand, pesticide residues in products cause potential harm to health and lead to environmental pollution. In recent years, the use of microorganisms to degrade pesticide residues has made great progress. However, at present, pesticide-degrading bacteria are mainly screened from soil and water sludge with serious pesticide pollution, and are mainly used to solve pesticide pollution in soil and water. Most of the screened strains are Pseudomonas and Klebsiella, etc. , the safety of the strain has been questioned, and its application is also subject to certai...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01
CPCA62D3/02C12N1/205C12R2001/01
Inventor 陈晶瑜曹明庞晓娜韩北忠
Owner CHINA AGRI UNIV
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