Induced strain (IJUV-6) of isaria javanica and dinotefuran compounded pesticide and applications thereof

A technology of IJUV-6 and insecticide, applied in the field of biological control, can solve problems such as increased drug resistance, achieve the effects of reducing the amount and frequency of use, slowing down the growth rate, and good compatibility

Active Publication Date: 2020-02-28
韶关市华实现代农业创新研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, the extensive use of dinotefuran and other pesticides on rice has led to a significant increase in the resistance of rice pests such as rice planthoppers and rice leafhoppers.

Method used

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  • Induced strain (IJUV-6) of isaria javanica and dinotefuran compounded pesticide and applications thereof
  • Induced strain (IJUV-6) of isaria javanica and dinotefuran compounded pesticide and applications thereof
  • Induced strain (IJUV-6) of isaria javanica and dinotefuran compounded pesticide and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The acquisition of embodiment 1 Isyriatella javanica mutagenic strain IJUV-6

[0033] The team of inventors collected brown planthopper [Nilaparvatalugens (Stal)] infested by insect fungus from rice in Guangzhou area, and obtained a plant with good control effect on rice planthopper and rice leafhopper after separation and screening from the insect corpse. Javanese IJNL-N8 (preservation number: CCTCC NO: M 2017709). In the later field application, it was found that the control effect of the strain on rice pests such as planthoppers and rice leafhoppers was unstable, and it was easily affected by environmental conditions such as strong ultraviolet rays and high temperatures in the field. To this end, we irradiated the wild strain (I. javanica IJNL-N8) with ultraviolet rays to induce its mutation. After screening and purification, we obtained the UV-resistant and high-temperature-resistant mutagenic strain IJUV-6 of I. javanica, which was released in 2019. It was deposit...

Embodiment 2

[0034] Example 2 Determination of the compatibility of Isyriatella javanica mutant strain IJUV-6 to dinotefuran

[0035] 1. Experimental method

[0036] Inoculate the UV-resistant I. javanica mutagenic strain IJUV-6 into Chase's medium (30.0 g of sucrose, sodium nitrate (NaNO 3 ) 3.0g, dipotassium hydrogen phosphate (K 2 HPO 4 ) 1.0g, magnesium sulfate (MgSO 4 .7H 2 O) 0.5g, potassium chloride (KCl) 0.5g, ferrous sulfate (FeSO 4 ) 0.01g, agar powder 20g, add water to make the total volume 1000mL) on a plate, culture in a constant temperature box (L:D=14:10) at 26±1°C for 10 days, then use 0.1% Tween-80 sterile water Collect the spores, mix the fungal spores and dinotefuran in Cha's culture medium, prepare suspensions containing different concentrations of dinotefuran (0.1x, 0.5x, 1x) and fungal spores, and test with the slide germination method;

[0037] Drop the above dinotefuran and spore suspension containing different concentrations on a sterile glass slide, place it...

Embodiment 3

[0043] Example 3 High temperature resistance and anti-ultraviolet performance test of I. javanica mutagenic strain IJUV-6

[0044] 1. Experimental method

[0045] The conidia of the wild strain (WT) and the mutant strain (UV) were respectively inoculated into PDA plates and cultivated until sporulation for later use, and the conidia of the above two strains were collected with 0.1% Tween-80 sterile water respectively Prepare a spore suspension of 1.0×106 spores / mL, place it under ultraviolet radiation for 0, 40min, then take 5mL of the spore suspension and add it to a Erlenmeyer flask filled with 200mL of Chase culture medium, at 26±1°C, 200rpm Under culture, observe and record the spore germination at 10h, 12h, and 14h, take the germ tube length exceeding half of the spore diameter as the germination standard, and repeat all treatments 3 times; by comparing the spore germination rate of each strain at 10h, 12h, and 14h In order to clarify the UV resistance of IJUV-6 mutageni...

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Abstract

The invention discloses an induced strain (IJUV-6) of isaria javanica and dinotefuran compounded pesticide and applications thereof. The invention at first provides an induced strain (IJUV-6) of isaria javanica and dinotefuran compounded pesticide and applications thereof. The compounded pesticide comprises an induced strain (IJUV-6) of isaria javanica and dinotefuran. The induced strain (IJUV-6)of isaria javanica is well compatible with dinotefuran. Dinotefuran does not influence the germination and growth of mycelia of the induced strain (IJUV-6). Low dosage dinotefuran and the induced strain (IJUV-6) are compounded to generate a synergistic effect, the control effect on rice plant-hoppers and rice leaf-hoppers is prominently enhanced, the using amount of dinotefuran is reduced, and thedrug resistance of rice plant-hoppers and rice leaf-hoppers is weakened. Moreover, the compounded pesticide has a stable control effect on rice plant-hoppers and rice leaf-hoppers under the conditions of strong UV rays and high temperature. The pesticide is pollution-free and no residue is generated. The pesticide has a wide application prospect in the control of rice plant-hoppers and rice leaf-hoppers.

Description

technical field [0001] The invention belongs to the technical field of biological control. More specifically, it relates to a compound insecticide of IJUV-6 and dinotefuran and its application. Background technique [0002] Rice planthopper and rice leafhopper are migratory pests on rice. Rice planthoppers migrate back and forth with air currents in the rice planting areas of southern and central my country in spring and autumn every year. In addition to directly stabbing and sucking rice juice and other damages, the more serious damage is the spread of rice virus diseases. The dwarf disease caused by the tillering and jointing stages can lead to a large area of ​​rice yield reduction of more than 50%, or even no harvest. In terms of control, chemical pesticides such as dinotefuran, thiamethoxam, imidacloprid, and pymetrozine are often used for control; Extensive use of chemical pesticides kills the natural enemies of pests, destroys the self-regulation ability of the rice...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/30A01N51/00A01P7/04
CPCA01N51/00
Inventor 刘光华黄振赵倩叶露
Owner 韶关市华实现代农业创新研究院
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